{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["11"],"submitter":["Liu T"],"funding":["National Natural Science Foundation of China"],"pubmed_abstract":["We first performed a combination of metabolic engineering (deletion of <i>ldh</i> and <i>poxB</i> and overexpression of <i>mmc</i>) with evolutionary engineering (selection under oxygen stress, acid stress and osmotic stress) in <i>Propionibacterium acidipropionici</i>. The results indicated that the mutants had superior physiological activity, especially the mutant III obtained from <i>P. acidipropionici-</i>Δ<i>ldh</i>-Δ<i>poxB</i>+<i>mmc</i> by evolutionary engineering, with 1.5-3.5 times higher growth rates, as well as a 37.1% increase of propionic acid (PA) titer and a 37.8% increase PA productivity compared to the wild type. Moreover, the integrative transcriptomics and proteomics analyses revealed that the differentially expressed genes (DEGs) and proteins (DEPs) in the mutant III w"],"journal":["Frontiers in microbiology"],"pagination":["1564"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC7438477"],"repository":["biostudies-literature"],"pubmed_title":["Transcriptomics and Proteomics Analyses of the Responses of <i>Propionibacterium acidipropionici</i> to Metabolic and Evolutionary Manipulation."],"pmcid":["PMC7438477"],"pubmed_authors":["Li Y","Jiang L","Zhao Q","Zhu L","Huang H","Liu T"],"additional_accession":[]},"is_claimable":false,"name":"Transcriptomics and Proteomics Analyses of the Responses of <i>Propionibacterium acidipropionici</i> to Metabolic and Evolutionary Manipulation.","description":"We first performed a combination of metabolic engineering (deletion of <i>ldh</i> and <i>poxB</i> and overexpression of <i>mmc</i>) with evolutionary engineering (selection under oxygen stress, acid stress and osmotic stress) in <i>Propionibacterium acidipropionici</i>. The results indicated that the mutants had superior physiological activity, especially the mutant III obtained from <i>P. acidipropionici-</i>Δ<i>ldh</i>-Δ<i>poxB</i>+<i>mmc</i> by evolutionary engineering, with 1.5-3.5 times higher growth rates, as well as a 37.1% increase of propionic acid (PA) titer and a 37.8% increase PA productivity compared to the wild type. Moreover, the integrative transcriptomics and proteomics analyses revealed that the differentially expressed genes (DEGs) and proteins (DEPs) in the mutant III w","dates":{"release":"2020-01-01T00:00:00Z","publication":"2020","modification":"2026-05-03T22:13:42.024Z","creation":"2020-09-13T07:03:27Z"},"accession":"S-EPMC7438477","cross_references":{"pubmed":["32903527"],"doi":["10.3389/fmicb.2020.01564"]}}