biostudies-literatureUnknown11Liu TNational Natural Science Foundation of ChinaWe first performed a combination of metabolic engineering (deletion of <i>ldh</i> and <i>poxB</i> and overexpression of <i>mmc</i>) with evolutionary engineering (selection under oxygen stress, acid stress and osmotic stress) in <i>Propionibacterium acidipropionici</i>. The results indicated that the mutants had superior physiological activity, especially the mutant III obtained from <i>P. acidipropionici-</i>Δ<i>ldh</i>-Δ<i>poxB</i>+<i>mmc</i> by evolutionary engineering, with 1.5-3.5 times higher growth rates, as well as a 37.1% increase of propionic acid (PA) titer and a 37.8% increase PA productivity compared to the wild type. Moreover, the integrative transcriptomics and proteomics analyses revealed that the differentially expressed genes (DEGs) and proteins (DEPs) in the mutant III wFrontiers in microbiology1564https://www.ebi.ac.uk/biostudies/studies/S-EPMC7438477biostudies-literatureTranscriptomics and Proteomics Analyses of the Responses of <i>Propionibacterium acidipropionici</i> to Metabolic and Evolutionary Manipulation.PMC7438477Li YJiang LZhao QZhu LHuang HLiu TfalseTranscriptomics and Proteomics Analyses of the Responses of <i>Propionibacterium acidipropionici</i> to Metabolic and Evolutionary Manipulation.We first performed a combination of metabolic engineering (deletion of <i>ldh</i> and <i>poxB</i> and overexpression of <i>mmc</i>) with evolutionary engineering (selection under oxygen stress, acid stress and osmotic stress) in <i>Propionibacterium acidipropionici</i>. The results indicated that the mutants had superior physiological activity, especially the mutant III obtained from <i>P. acidipropionici-</i>Δ<i>ldh</i>-Δ<i>poxB</i>+<i>mmc</i> by evolutionary engineering, with 1.5-3.5 times higher growth rates, as well as a 37.1% increase of propionic acid (PA) titer and a 37.8% increase PA productivity compared to the wild type. Moreover, the integrative transcriptomics and proteomics analyses revealed that the differentially expressed genes (DEGs) and proteins (DEPs) in the mutant III w2020-01-01T00:00:00Z20202026-05-03T22:13:42.024Z2020-09-13T07:03:27ZS-EPMC74384773290352710.3389/fmicb.2020.01564