biostudies-literatureUnknown47(4)Gassner CThe U antigen (MNS5) is one of 49 antigens belonging to the MNS blood group system (ISBT002) carried on glycophorins A (GPA) and B (GPB). U is present on the red blood cells in almost all Europeans and Asians but absent in approximately 1.0% of Black Africans. U negativity coincides with negativity for S (MNS3) and s (MNS4) on GPB, thus be called S-s-U-, and is thought to arise from homozygous deletion of <i>GYPB</i>. Little is known about the molecular background of these deletions. Bioinformatic analysis of the 1000 Genomes Project data revealed several candidate regions with apparent deletions in <i>GYPB</i>. Highly specific Gap-PCRs, only resulting in positive amplification from DNAs with deletions present, allowed for the exact genetic localization of 3 different breakpoints; 110.24- Transfusion medicine and hemotherapy : offizielles Organ der Deutschen Gesellschaft fur Transfusionsmedizin und Immunhamatologie326-336https://www.ebi.ac.uk/biostudies/studies/S-EPMC7443675biostudies-literatureTwo Prevalent ∼100-kb <i>GYPB</i> Deletions Causative of the GPB-Deficient Blood Group MNS Phenotype S-s-U- in Black Africans.PMC7443675Just BTrost NFrey BMForster MFranke ABensing KMStorry JRGassner CMeyer SEngstrom CDenomme GASong YLJungbauer CPortmann CMattle-Greminger MPfalseTwo Prevalent ∼100-kb <i>GYPB</i> Deletions Causative of the GPB-Deficient Blood Group MNS Phenotype S-s-U- in Black Africans.The U antigen (MNS5) is one of 49 antigens belonging to the MNS blood group system (ISBT002) carried on glycophorins A (GPA) and B (GPB). U is present on the red blood cells in almost all Europeans and Asians but absent in approximately 1.0% of Black Africans. U negativity coincides with negativity for S (MNS3) and s (MNS4) on GPB, thus be called S-s-U-, and is thought to arise from homozygous deletion of <i>GYPB</i>. Little is known about the molecular background of these deletions. Bioinformatic analysis of the 1000 Genomes Project data revealed several candidate regions with apparent deletions in <i>GYPB</i>. Highly specific Gap-PCRs, only resulting in positive amplification from DNAs with deletions present, allowed for the exact genetic localization of 3 different breakpoints; 110.24- 2020-01-01T00:00:00Z2020 Jul2026-06-02T16:17:55.383Z2020-09-05T07:09:35ZS-EPMC74436753288450510.1159/000504946