{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Sangesland M"],"funding":["NIDA NIH HHS","NIDDK NIH HHS","NIAID NIH HHS","National Institutes of Health","NIGMS NIH HHS"],"pagination":["108065"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC7446668"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["32(8)"],"pubmed_abstract":["B cell receptors (BCRs) display a combination of variable (V)-gene-encoded complementarity determining regions (CDRs) and adaptive/hypervariable CDR3 loops to engage antigens. It has long been proposed that the former tune for recognition of pathogens or groups of pathogens. To experimentally evaluate this within the human antibody repertoire, we perform immune challenges in transgenic mice that bear diverse human CDR3 and light chains but are constrained to different human V<sub>H</sub><sub>-</sub>genes. We find that, of six commonly deployed V<sub>H</sub> sequences, only those CDRs encoded by IGHV1-2<sup>∗</sup>02 enable polyclonal antibody responses against bacterial lipopolysaccharide (LPS) when introduced to the bloodstream. The LPS is from diverse strains of gram-negative bacteria, a"],"journal":["Cell reports"],"pubmed_title":["A Single Human V<sub>H</sub>-gene Allows for a Broad-Spectrum Antibody Response Targeting Bacterial Lipopolysaccharides in the Blood."],"pmcid":["PMC7446668"],"funding_grant_id":["DP2 DA042422","R01 AI155447","U24 AI118672","DP2 GM119419","U19 AI057229","R01 AI124378","R01 DK120485","F31 AI138368","R01 AI137057"],"pubmed_authors":["Barnes RM","Lingwood D","Zhu AL","Gatter GJ","Hayward MR","Quirindongo-Crespo M","Lonberg N","Kwon D","Kazer SW","Shalek AK","Yousif AS","Ronsard L","Rohrer D","Sangesland M"],"additional_accession":[]},"is_claimable":false,"name":"A Single Human V<sub>H</sub>-gene Allows for a Broad-Spectrum Antibody Response Targeting Bacterial Lipopolysaccharides in the Blood.","description":"B cell receptors (BCRs) display a combination of variable (V)-gene-encoded complementarity determining regions (CDRs) and adaptive/hypervariable CDR3 loops to engage antigens. It has long been proposed that the former tune for recognition of pathogens or groups of pathogens. To experimentally evaluate this within the human antibody repertoire, we perform immune challenges in transgenic mice that bear diverse human CDR3 and light chains but are constrained to different human V<sub>H</sub><sub>-</sub>genes. We find that, of six commonly deployed V<sub>H</sub> sequences, only those CDRs encoded by IGHV1-2<sup>∗</sup>02 enable polyclonal antibody responses against bacterial lipopolysaccharide (LPS) when introduced to the bloodstream. The LPS is from diverse strains of gram-negative bacteria, a","dates":{"release":"2020-01-01T00:00:00Z","publication":"2020 Aug","modification":"2025-04-19T04:02:26.217Z","creation":"2020-08-31T07:04:35Z"},"accession":"S-EPMC7446668","cross_references":{"pubmed":["32846123"],"doi":["10.1016/j.celrep.2020.108065"]}}