biostudies-literatureYang Pthe Young Scientists Fund of the National Natural Science Foundation of Chinathe National Natural Science Foundation of Chinae0237164https://www.ebi.ac.uk/biostudies/studies/S-EPMC7446844biostudies-literatureUnknown15(8)Minor grain crops are widely cultivated in northwest China and played important roles in local economic. Soil microbes play a central role in ecological function and biological stability and related to soil quality. In order to uncover the soil microbial composition differences and the factors under 5-year continuous monocropping of three minor crops (Proso millet, Common bean and Common buckwheat) in Guan-Zhong Plain, six soil nutrimental parameters, soil pH, soil moisture content, and four soil enzyme activities were analyzed and soil microbial composition were sequenced. The results showed that after 5-years of continuous monocropping, different cover crops influenced most of soil physicochemical properties, expect soil moisture content (P < 0.05), the available nutrients were significaPloS oneSoil properties, bacterial and fungal community compositions and the key factors after 5-year continuous monocropping of three minor crops.PMC74468443150136531371529Wang PYang PGao YGao JLuo YGao XFeng BfalseSoil properties, bacterial and fungal community compositions and the key factors after 5-year continuous monocropping of three minor crops.Minor grain crops are widely cultivated in northwest China and played important roles in local economic. Soil microbes play a central role in ecological function and biological stability and related to soil quality. In order to uncover the soil microbial composition differences and the factors under 5-year continuous monocropping of three minor crops (Proso millet, Common bean and Common buckwheat) in Guan-Zhong Plain, six soil nutrimental parameters, soil pH, soil moisture content, and four soil enzyme activities were analyzed and soil microbial composition were sequenced. The results showed that after 5-years of continuous monocropping, different cover crops influenced most of soil physicochemical properties, expect soil moisture content (P < 0.05), the available nutrients were significa2020-01-01T00:00:00Z20202025-04-19T14:57:40.289Z2020-08-30T07:22:07ZS-EPMC74468443283398010.1371/journal.pone.0237164