biostudies-literatureUnknown2020Li Y<h4>Background</h4>Tissue-resident macrophages can be educated to tumor-associated macrophages (TAMs) by the tumor microenvironment and many types of macrophages express erythropoietic receptor (EPOR); However, little is known about the expression of EPOR on TAMs and the identity of EPOR⁺ TAMs in osteosarcoma lung metastasis has thus far remained elusive.<h4>Methods</h4>EPOR-eGFPcre mice were used to determine the expression of EPOR on lung tissue-resident macrophages. Flow cytometry, RT-PCR, and Western blot were examined to define the identity of EPOR⁺ TAMs in 106 osteosarcoma lung metastasis specimens. Moreover, the clinicopathologic factors and prognosis of patients with CD163⁺EPOR⁺ macrophages were compared.<h4>Results</h4>We found that a subpopulation of mouse lung tissue-resident macrophages express EPOR and EPO enhances the proliferation of EPOR⁺ macrophages in mouse lung. A subpopulation of CD163⁺ macrophages expresses EPOR in human osteosarcoma lung metastasis specimens. CD163⁺EPOR⁺TAMs increase 2.5 times in human osteosarcoma lung metastasis tissues; CD206, CD163, and PD1, which are known to have a significant role in TAM function had high expression in CD163⁺EPOR⁺ TAMs compared with CD163⁺EPOR^- TAMs. Furthermore, CD163⁺EPOR⁺ TAMs had higher M2 marker and cytokine expression in osteosarcoma tissues compared with para-osteosarcoma tissues. EPO enhanced the expression of M2 cytokines in primary CD163⁺EPOR⁺ TAMs. Importantly, the percentage of CD163⁺EPOR⁺ TAMs had a positive linear association with malignant phenotypes as well as poor disease-free survival and overall survival time.<h4>Conclusions</h4>We have characterized TAMs expressing EPOR and CD163⁺EPOR⁺ macrophages as TAMs in osteosarcoma lung metastasis patients, which are highly associated with tumor aggressiveness.Journal of immunology research9374240https://www.ebi.ac.uk/biostudies/studies/S-EPMC7450330biostudies-literatureIdentification and Functional Analysis of EPOR⁺ Tumor-Associated Macrophages in Human Osteosarcoma Lung Metastasis.PMC7450330Wu JLi YWei RLi MfalseIdentification and Functional Analysis of EPOR⁺ Tumor-Associated Macrophages in Human Osteosarcoma Lung Metastasis.<h4>Background</h4>Tissue-resident macrophages can be educated to tumor-associated macrophages (TAMs) by the tumor microenvironment and many types of macrophages express erythropoietic receptor (EPOR); However, little is known about the expression of EPOR on TAMs and the identity of EPOR⁺ TAMs in osteosarcoma lung metastasis has thus far remained elusive.<h4>Methods</h4>EPOR-eGFPcre mice were used to determine the expression of EPOR on lung tissue-resident macrophages. Flow cytometry, RT-PCR, and Western blot were examined to define the identity of EPOR⁺ TAMs in 106 osteosarcoma lung metastasis specimens. Moreover, the clinicopathologic factors and prognosis of patients with CD163⁺EPOR⁺ macrophages were compared.<h4>Results</h4>We found that a subpopulation of mouse lung tissue-resident macrophages express EPOR and EPO enhances the proliferation of EPOR⁺ macrophages in mouse lung. A subpopulation of CD163⁺ macrophages expresses EPOR in human osteosarcoma lung metastasis specimens. CD163⁺EPOR⁺TAMs increase 2.5 times in human osteosarcoma lung metastasis tissues; CD206, CD163, and PD1, which are known to have a significant role in TAM function had high expression in CD163⁺EPOR⁺ TAMs compared with CD163⁺EPOR^- TAMs. Furthermore, CD163⁺EPOR⁺ TAMs had higher M2 marker and cytokine expression in osteosarcoma tissues compared with para-osteosarcoma tissues. EPO enhanced the expression of M2 cytokines in primary CD163⁺EPOR⁺ TAMs. Importantly, the percentage of CD163⁺EPOR⁺ TAMs had a positive linear association with malignant phenotypes as well as poor disease-free survival and overall survival time.<h4>Conclusions</h4>We have characterized TAMs expressing EPOR and CD163⁺EPOR⁺ macrophages as TAMs in osteosarcoma lung metastasis patients, which are highly associated with tumor aggressiveness.2020-01-01T00:00:00Z20202024-02-14T23:49:08.987Z2020-09-12T07:07:03ZS-EPMC74503303290894210.1155/2020/9374240