biostudies-literatureAbdi KIntramural NIH HHS415-419https://www.ebi.ac.uk/biostudies/studies/S-EPMC7454002biostudies-literatureUnknown4(7)Dendritic cells (DCs) play a key role in the initiation of an immune response and are known as "professional" APCs because of their ability to activate naive T cells. A widely used method to generate DCs in vitro is to culture bone marrow (BM) cells or blood monocytes in the presence of GM-CSF and IL-4. In this study, we show that a small population of NK cells residing in the BM of RAG^-/-, but not RAG^-/- γ_c chain^-/- mice, remain in the DC culture and is the source of IFN-γ produced after stimulation with LPS. These cells, which may represent early promoters of LPS-induced responses, have to be taken into account when interpreting experiments using BM-derived DCs.ImmunoHorizonsBone Marrow-Derived Dendritic Cell Cultures from RAG^-/- Mice Include IFN-γ-Producing NK Cells.PMC7454002Z99 AI999999Thomas LMLaky KMatzinger PAbshari MAbdi KLong EOfalseBone Marrow-Derived Dendritic Cell Cultures from RAG^-/- Mice Include IFN-γ-Producing NK Cells.Dendritic cells (DCs) play a key role in the initiation of an immune response and are known as "professional" APCs because of their ability to activate naive T cells. A widely used method to generate DCs in vitro is to culture bone marrow (BM) cells or blood monocytes in the presence of GM-CSF and IL-4. In this study, we show that a small population of NK cells residing in the BM of RAG^-/-, but not RAG^-/- γ_c chain^-/- mice, remain in the DC culture and is the source of IFN-γ produced after stimulation with LPS. These cells, which may represent early promoters of LPS-induced responses, have to be taken into account when interpreting experiments using BM-derived DCs.2020-01-01T00:00:00Z2020 Jul2024-11-21T07:57:12.982Z2020-08-31T07:30:21ZS-EPMC74540023266530010.4049/immunohorizons.2000011