{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Wang L"],"funding":["Ministry of Science and Technology of the People&apos;s Republic of China","National Science Foundation of China | National Natural Science Foundation of China-Yunnan Joint Fund","National Program on Key Research Project;"],"pagination":["14298"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC7459345"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["10(1)"],"pubmed_abstract":["Atmospheric and room-temperature plasma (ARTP) has been successfully developed as a useful mutation tool for mutation breeding of various microbes and plants as well animals by genetic alterations. However, understanding of the molecular mechanisms underlying the biological responses to ARTP irradiation is still limited. Therefore, to gain a molecular understanding of how irradiation with ARTP damages DNA, we irradiated the artificially synthesized mononucleotides of dATP, dTTP, dGTP, and dCTP, and the oligonucleotides of dA<sub>8</sub>, dT<sub>8</sub>, dG<sub>8</sub>, dC<sub>8</sub>, and dA<sub>2</sub>dT<sub>2</sub>dG<sub>2</sub>dC<sub>2</sub> as chemical building blocks of DNA with ARTP for 1-4 min, identified the mononucleotide products using <sup>31</sup>P- and <sup>1</sup>H-nuclear ma"],"journal":["Scientific reports"],"pubmed_title":["Insights into the molecular-level effects of atmospheric and room-temperature plasma on mononucleotides and single-stranded homo- and hetero-oligonucleotides."],"pmcid":["PMC7459345"],"funding_grant_id":["21627812","2016YFD0102106"],"pubmed_authors":["Li G","Li H","He D","Su N","Xing XH","Wu Y","Wang L","Zhao H","Jin L"],"additional_accession":[]},"is_claimable":false,"name":"Insights into the molecular-level effects of atmospheric and room-temperature plasma on mononucleotides and single-stranded homo- and hetero-oligonucleotides.","description":"Atmospheric and room-temperature plasma (ARTP) has been successfully developed as a useful mutation tool for mutation breeding of various microbes and plants as well animals by genetic alterations. However, understanding of the molecular mechanisms underlying the biological responses to ARTP irradiation is still limited. Therefore, to gain a molecular understanding of how irradiation with ARTP damages DNA, we irradiated the artificially synthesized mononucleotides of dATP, dTTP, dGTP, and dCTP, and the oligonucleotides of dA<sub>8</sub>, dT<sub>8</sub>, dG<sub>8</sub>, dC<sub>8</sub>, and dA<sub>2</sub>dT<sub>2</sub>dG<sub>2</sub>dC<sub>2</sub> as chemical building blocks of DNA with ARTP for 1-4 min, identified the mononucleotide products using <sup>31</sup>P- and <sup>1</sup>H-nuclear ma","dates":{"release":"2020-01-01T00:00:00Z","publication":"2020 Aug","modification":"2025-04-03T22:39:44.088Z","creation":"2020-09-05T07:09:51Z"},"accession":"S-EPMC7459345","cross_references":{"pubmed":["32868795"],"doi":["10.1038/s41598-020-71152-1"]}}