biostudies-literatureIto HJapan Society for the Promotion of ScienceE1375https://www.ebi.ac.uk/biostudies/studies/S-EPMC7459485biostudies-literatureUnknown10(8)The Tsushima leopard cat (Prionailurus bengalensis euptilurus) lives on Tsushima Island in Japan and is a regional population of the Amur leopard cat; it is threatened with extinction. Its genetic management is important because of the small population. We used genotyping by random amplicon sequencing-direct (GRAS-Di) to develop a draft genome and explore single-nucleotide polymorphism (SNP) markers. The SNPs were analyzed using three genotyping methods (mapping de novo, to the Tsushima leopard cat draft genome, and to the domestic cat genome). We examined the genetic diversity and genetic structure of the Tsushima leopard cat. The genome size was approximately 2.435 Gb. The number of SNPs identified was 133-158. The power of these markers was sufficient for individual and parentage identiAnimals : an open access journal from MDPIGenetic Diversity and Genetic Structure of the Wild Tsushima Leopard Cat from Genome-Wide Analysis.PMC745948520H0042016H0689219K15861Onuma MMurayama MIto HNakajima NfalseGenetic Diversity and Genetic Structure of the Wild Tsushima Leopard Cat from Genome-Wide Analysis.The Tsushima leopard cat (Prionailurus bengalensis euptilurus) lives on Tsushima Island in Japan and is a regional population of the Amur leopard cat; it is threatened with extinction. Its genetic management is important because of the small population. We used genotyping by random amplicon sequencing-direct (GRAS-Di) to develop a draft genome and explore single-nucleotide polymorphism (SNP) markers. The SNPs were analyzed using three genotyping methods (mapping de novo, to the Tsushima leopard cat draft genome, and to the domestic cat genome). We examined the genetic diversity and genetic structure of the Tsushima leopard cat. The genome size was approximately 2.435 Gb. The number of SNPs identified was 133-158. The power of these markers was sufficient for individual and parentage identi2020-01-01T00:00:00Z2020 Aug2025-04-26T13:33:50.312Z2020-09-06T07:07:00ZS-EPMC74594853278478210.3390/ani10081375