{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Ndzeshang LB"],"funding":["German Research Council","Bill and Melinda Gates Foundation"],"pagination":["e0008415"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC7470323"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["14(8)"],"pubmed_abstract":["Basic and translational research on loiasis, a filarial nematode infection of medical importance, is impeded by a lack of suitable Loa loa infection models and techniques of obtaining and culturing life cycle stages. We describe the development of a new method for routine production of infective third-stage larvae (L3) of L. loa from the natural intermediate arthropod vector host, Chrysops silacea, following experimental infection with purified microfilariae. At 14-days post-infection of C. silacea, the fly survival rate was 43%. Survival was significantly higher in flies injected with 50 mf (55.2%) than those that received 100 mf (31.0%). However, yield per surviving fly and total yield of L3 was markedly higher in the group of flies inoculated with 100 mf (3474 vs 2462 L3 produced). The "],"journal":["PLoS neglected tropical diseases"],"pubmed_title":["Generation of Loa loa infective larvae by experimental infection of the vector, Chrysops silacea."],"pmcid":["PMC7470323"],"funding_grant_id":["DFG RI 3036/1-1","OPP1119043"],"pubmed_authors":["Fombad FF","Steven A","Pionnier NP","Njouendou AJ","Ritter M","Layland LE","Gandjui NVT","Turner JD","Chunda VC","Taylor MJ","Akumtoh DN","Chounna PWN","Hoerauf A","Wanji S","Ndzeshang LB"],"additional_accession":[]},"is_claimable":false,"name":"Generation of Loa loa infective larvae by experimental infection of the vector, Chrysops silacea.","description":"Basic and translational research on loiasis, a filarial nematode infection of medical importance, is impeded by a lack of suitable Loa loa infection models and techniques of obtaining and culturing life cycle stages. We describe the development of a new method for routine production of infective third-stage larvae (L3) of L. loa from the natural intermediate arthropod vector host, Chrysops silacea, following experimental infection with purified microfilariae. At 14-days post-infection of C. silacea, the fly survival rate was 43%. Survival was significantly higher in flies injected with 50 mf (55.2%) than those that received 100 mf (31.0%). However, yield per surviving fly and total yield of L3 was markedly higher in the group of flies inoculated with 100 mf (3474 vs 2462 L3 produced). The ","dates":{"release":"2020-01-01T00:00:00Z","publication":"2020 Aug","modification":"2025-04-19T15:08:05.72Z","creation":"2020-09-14T07:13:55Z"},"accession":"S-EPMC7470323","cross_references":{"pubmed":["32804951"],"doi":["10.1371/journal.pntd.0008415"]}}