biostudies-literatureNdzeshang LBGerman Research CouncilBill and Melinda Gates Foundatione0008415https://www.ebi.ac.uk/biostudies/studies/S-EPMC7470323biostudies-literatureUnknown14(8)Basic and translational research on loiasis, a filarial nematode infection of medical importance, is impeded by a lack of suitable Loa loa infection models and techniques of obtaining and culturing life cycle stages. We describe the development of a new method for routine production of infective third-stage larvae (L3) of L. loa from the natural intermediate arthropod vector host, Chrysops silacea, following experimental infection with purified microfilariae. At 14-days post-infection of C. silacea, the fly survival rate was 43%. Survival was significantly higher in flies injected with 50 mf (55.2%) than those that received 100 mf (31.0%). However, yield per surviving fly and total yield of L3 was markedly higher in the group of flies inoculated with 100 mf (3474 vs 2462 L3 produced). The PLoS neglected tropical diseasesGeneration of Loa loa infective larvae by experimental infection of the vector, Chrysops silacea.PMC7470323DFG RI 3036/1-1OPP1119043Fombad FFSteven APionnier NPNjouendou AJRitter MLayland LEGandjui NVTTurner JDChunda VCTaylor MJAkumtoh DNChounna PWNHoerauf AWanji SNdzeshang LBfalseGeneration of Loa loa infective larvae by experimental infection of the vector, Chrysops silacea.Basic and translational research on loiasis, a filarial nematode infection of medical importance, is impeded by a lack of suitable Loa loa infection models and techniques of obtaining and culturing life cycle stages. We describe the development of a new method for routine production of infective third-stage larvae (L3) of L. loa from the natural intermediate arthropod vector host, Chrysops silacea, following experimental infection with purified microfilariae. At 14-days post-infection of C. silacea, the fly survival rate was 43%. Survival was significantly higher in flies injected with 50 mf (55.2%) than those that received 100 mf (31.0%). However, yield per surviving fly and total yield of L3 was markedly higher in the group of flies inoculated with 100 mf (3474 vs 2462 L3 produced). The 2020-01-01T00:00:00Z2020 Aug2025-04-19T15:08:05.72Z2020-09-14T07:13:55ZS-EPMC74703233280495110.1371/journal.pntd.0008415