{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Nishio T"],"funding":["Japan Society for the Promotion of Science"],"pagination":["e0238447"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC7470421"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["15(9)"],"pubmed_abstract":["<h4>Background</h4>Polyamines are involved in a wide variety of biological processes including a marked effect on the structure and function of DNA. During our study on the interaction of polyamines with DNA, we found that K+ enhanced in vitro gene expression in the presence of polyamine more strongly than Na+. Thus, we sought to clarify the physico-chemical mechanism underlying this marked difference between the effects of K+ and Na+.<h4>Principal findings</h4>It was found that K+ enhanced gene expression in the presence of spermidine, SPD(3+), much more strongly than Na+, through in vitro experiments with a Luciferase assay on cell extracts. Single-DNA observation by fluorescence microscopy showed that Na+ prevents the folding transition of DNA into a compact state more strongly than K+."],"journal":["PloS one"],"pubmed_title":["K+ promotes the favorable effect of polyamine on gene expression better than Na."],"pmcid":["PMC7470421"],"funding_grant_id":["JP20H01877"],"pubmed_authors":["Yoshikawa Y","Sadakane K","Nishio T","Sugino K","Oe Y","Yoshikawa K","Matsumoto M"],"additional_accession":[]},"is_claimable":false,"name":"K+ promotes the favorable effect of polyamine on gene expression better than Na.","description":"<h4>Background</h4>Polyamines are involved in a wide variety of biological processes including a marked effect on the structure and function of DNA. During our study on the interaction of polyamines with DNA, we found that K+ enhanced in vitro gene expression in the presence of polyamine more strongly than Na+. Thus, we sought to clarify the physico-chemical mechanism underlying this marked difference between the effects of K+ and Na+.<h4>Principal findings</h4>It was found that K+ enhanced gene expression in the presence of spermidine, SPD(3+), much more strongly than Na+, through in vitro experiments with a Luciferase assay on cell extracts. Single-DNA observation by fluorescence microscopy showed that Na+ prevents the folding transition of DNA into a compact state more strongly than K+.","dates":{"release":"2020-01-01T00:00:00Z","publication":"2020","modification":"2025-05-29T19:37:25.551Z","creation":"2025-05-29T19:37:25.551Z"},"accession":"S-EPMC7470421","cross_references":{"pubmed":["32881909"],"doi":["10.1371/journal.pone.0238447"]}}