biostudies-literatureUnknown11Szamosi JC<h4>Background</h4>In studies evaluating the microbiome, numerous factors can contribute to technical variability. These factors include DNA extraction methodology, sequencing protocols, and data analysis strategies. We sought to evaluate the impact these factors have on the results obtained when the sequence data are independently generated and analyzed by different laboratories.<h4>Methods</h4>To evaluate the effect of technical variability, we used human intestinal biopsy samples resected from individuals diagnosed with an inflammatory bowel disease (IBD), including Crohn's disease (<i>n</i> = 12) and ulcerative colitis (<i>n</i> = 10), and those without IBD (<i>n</i> = 10). Matched samples from each participant were sent to three laboratories and studied using independent protocols forFrontiers in microbiology2028https://www.ebi.ac.uk/biostudies/studies/S-EPMC7472644biostudies-literatureAssessment of Inter-Laboratory Variation in the Characterization and Analysis of the Mucosal Microbiota in Crohn's Disease and Ulcerative Colitis.PMC7472644Bernstein CNKnox NCSzamosi JCVan Domselaar GCopeland JKBonner CGuttman DSRossi LShekarriz SGraham MForbes JDSurette MGfalseAssessment of Inter-Laboratory Variation in the Characterization and Analysis of the Mucosal Microbiota in Crohn's Disease and Ulcerative Colitis.<h4>Background</h4>In studies evaluating the microbiome, numerous factors can contribute to technical variability. These factors include DNA extraction methodology, sequencing protocols, and data analysis strategies. We sought to evaluate the impact these factors have on the results obtained when the sequence data are independently generated and analyzed by different laboratories.<h4>Methods</h4>To evaluate the effect of technical variability, we used human intestinal biopsy samples resected from individuals diagnosed with an inflammatory bowel disease (IBD), including Crohn's disease (<i>n</i> = 12) and ulcerative colitis (<i>n</i> = 10), and those without IBD (<i>n</i> = 10). Matched samples from each participant were sent to three laboratories and studied using independent protocols for2020-01-01T00:00:00Z20202026-04-07T17:43:53.072Z2020-09-30T07:06:37ZS-EPMC74726443297373410.3389/fmicb.2020.02028