biostudies-literatureBornhauser BSwiss National Science Foundation4052-4064https://www.ebi.ac.uk/biostudies/studies/S-EPMC7479947biostudies-literatureUnknown4(17)Most relapses of acute lymphoblastic leukemia (ALL) occur in patients with a medium risk (MR) for relapse on the Associazione Italiana di Ematologia e Oncologia Pediatrica and Berlin-Frankfurt-Münster (AIEOP-BFM) ALL protocol, based on persistence of minimal residual disease (MRD). New insights into biological features that are associated with MRD are needed. Here, we identify the glycosylphosphatidylinositol-anchored cell surface protein vanin-2 (VNN2; GPI-80) by charting the cell surface proteome of MRD very high-risk (HR) B-cell precursor (BCP) ALL using a chemoproteomics strategy. The correlation between VNN2 transcript and surface protein expression enabled a retrospective analysis (ALL-BFM 2000; N = 770 cases) using quantitative polymerase chain reaction to confirm the association ofBlood advancesThe hematopoietic stem cell marker VNN2 is associated with chemoresistance in pediatric B-cell precursor ALL.PMC7479947182269156407310030Wollscheid BZimmermann MTemperli MRodriguez Martinez VScheidegger NRinaldi AHauri-Hohl MMirkowska PBruggemann MGaipa GBasso GNgo QVendramini ESchrappe MDworzak MAttarbaschi APanzer-Grumayer RMarovca BEckert CMoller CBarz MJYaspo MLSchumich ABourquin JPMejstrikova ETe Kronnie GBuldini BScarparo PNiggli FWarnatz HJSchutte MSkroblyn TLBornhauser BRisch TRitgen MStanulla MHofmann AMaglia OCario GfalseThe hematopoietic stem cell marker VNN2 is associated with chemoresistance in pediatric B-cell precursor ALL.Most relapses of acute lymphoblastic leukemia (ALL) occur in patients with a medium risk (MR) for relapse on the Associazione Italiana di Ematologia e Oncologia Pediatrica and Berlin-Frankfurt-Münster (AIEOP-BFM) ALL protocol, based on persistence of minimal residual disease (MRD). New insights into biological features that are associated with MRD are needed. Here, we identify the glycosylphosphatidylinositol-anchored cell surface protein vanin-2 (VNN2; GPI-80) by charting the cell surface proteome of MRD very high-risk (HR) B-cell precursor (BCP) ALL using a chemoproteomics strategy. The correlation between VNN2 transcript and surface protein expression enabled a retrospective analysis (ALL-BFM 2000; N = 770 cases) using quantitative polymerase chain reaction to confirm the association of2020-01-01T00:00:00Z2020 Sep2026-04-15T09:03:33.118Z2020-09-12T07:08:46ZS-EPMC74799473285338210.1182/bloodadvances.2019000938