{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Andrews WT"],"funding":["NIA NIH HHS","National Institute of General Medical Sciences","NIH HHS","National Institute on Aging","NIGMS NIH HHS"],"pagination":["6275-6285"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC7483268"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["412(24)"],"pubmed_abstract":["The N-methyl-D-aspartate (NMDA) receptor is a crucial mediator of pathological glutamate-driven excitotoxicity and subsequent neuronal death in acute ischemic stroke. Although the roles of the NMDAR's composite GluN2A-C subunits have been investigated in this phenomenon, the relative importance of the GluN2D subunit has yet to be evaluated. Herein, GluN2D<sup>-/-</sup> mice were studied in a model of ischemic stroke using MALDI FT-ICR mass spectrometry imaging to investigate the role of the GluN2D subunit of the NMDA receptor in brain ischemia. GluN2D<sup>-/-</sup> mice underwent middle cerebral artery occlusion (MCAO) and brain tissue was subsequently harvested, frozen, and cryosectioned. Tissue sections were analyzed via MALDI FT-ICR mass spectrometry imaging. MALDI analyses revealed inc"],"journal":["Analytical and bioanalytical chemistry"],"pubmed_title":["In situ metabolite and lipid analysis of GluN2D<sup>-/-</sup> and wild-type mice after ischemic stroke using MALDI MSI."],"pmcid":["PMC7483268"],"funding_grant_id":["R21-AG062144","S10 OD018507","R01 GM110406","R21 AG062144"],"pubmed_authors":["Andrews WT","Holmes A","Castellino FJ","Hummon AB","Donahue D","Balsara R"],"additional_accession":[]},"is_claimable":false,"name":"In situ metabolite and lipid analysis of GluN2D<sup>-/-</sup> and wild-type mice after ischemic stroke using MALDI MSI.","description":"The N-methyl-D-aspartate (NMDA) receptor is a crucial mediator of pathological glutamate-driven excitotoxicity and subsequent neuronal death in acute ischemic stroke. Although the roles of the NMDAR's composite GluN2A-C subunits have been investigated in this phenomenon, the relative importance of the GluN2D subunit has yet to be evaluated. Herein, GluN2D<sup>-/-</sup> mice were studied in a model of ischemic stroke using MALDI FT-ICR mass spectrometry imaging to investigate the role of the GluN2D subunit of the NMDA receptor in brain ischemia. GluN2D<sup>-/-</sup> mice underwent middle cerebral artery occlusion (MCAO) and brain tissue was subsequently harvested, frozen, and cryosectioned. Tissue sections were analyzed via MALDI FT-ICR mass spectrometry imaging. MALDI analyses revealed inc","dates":{"release":"2020-01-01T00:00:00Z","publication":"2020 Sep","modification":"2025-04-04T00:38:25.944Z","creation":"2022-02-11T10:02:18.648Z"},"accession":"S-EPMC7483268","cross_references":{"pubmed":["32107573"],"doi":["10.1007/s00216-020-02477-z"]}}