biostudies-literatureAndrews WTNIA NIH HHSNational Institute of General Medical SciencesNIH HHSNational Institute on AgingNIGMS NIH HHS6275-6285https://www.ebi.ac.uk/biostudies/studies/S-EPMC7483268biostudies-literatureUnknown412(24)The N-methyl-D-aspartate (NMDA) receptor is a crucial mediator of pathological glutamate-driven excitotoxicity and subsequent neuronal death in acute ischemic stroke. Although the roles of the NMDAR's composite GluN2A-C subunits have been investigated in this phenomenon, the relative importance of the GluN2D subunit has yet to be evaluated. Herein, GluN2D^-/- mice were studied in a model of ischemic stroke using MALDI FT-ICR mass spectrometry imaging to investigate the role of the GluN2D subunit of the NMDA receptor in brain ischemia. GluN2D^-/- mice underwent middle cerebral artery occlusion (MCAO) and brain tissue was subsequently harvested, frozen, and cryosectioned. Tissue sections were analyzed via MALDI FT-ICR mass spectrometry imaging. MALDI analyses revealed incAnalytical and bioanalytical chemistryIn situ metabolite and lipid analysis of GluN2D^-/- and wild-type mice after ischemic stroke using MALDI MSI.PMC7483268R21-AG062144S10 OD018507R01 GM110406R21 AG062144Andrews WTHolmes ACastellino FJHummon ABDonahue DBalsara RfalseIn situ metabolite and lipid analysis of GluN2D^-/- and wild-type mice after ischemic stroke using MALDI MSI.The N-methyl-D-aspartate (NMDA) receptor is a crucial mediator of pathological glutamate-driven excitotoxicity and subsequent neuronal death in acute ischemic stroke. Although the roles of the NMDAR's composite GluN2A-C subunits have been investigated in this phenomenon, the relative importance of the GluN2D subunit has yet to be evaluated. Herein, GluN2D^-/- mice were studied in a model of ischemic stroke using MALDI FT-ICR mass spectrometry imaging to investigate the role of the GluN2D subunit of the NMDA receptor in brain ischemia. GluN2D^-/- mice underwent middle cerebral artery occlusion (MCAO) and brain tissue was subsequently harvested, frozen, and cryosectioned. Tissue sections were analyzed via MALDI FT-ICR mass spectrometry imaging. MALDI analyses revealed inc2020-01-01T00:00:00Z2020 Sep2025-04-04T00:38:25.944Z2022-02-11T10:02:18.648ZS-EPMC74832683210757310.1007/s00216-020-02477-z