{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Rios KE"],"funding":["National Institute of Allergy and Infectious Diseases","NIAID NIH HHS","National Institute of General Medical Sciences","NIGMS NIH HHS"],"pagination":["104179"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC7484395"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["356"],"pubmed_abstract":["After T cell receptor (TCR) engagement, the CARD11-Bcl10-Malt1 (CBM) complex oligomerizes to transduce NF-κB activating signals. Bcl10 is then degraded to limit NF-κB activation. The cDNA AK057716 (BinCARD-1) was reported to encode a novel CARD protein that interacts with Bcl10 and modestly inhibits NF-κB activation. In a later study, a second isoform, BinCARD-2, was identified. Here, we report that the cDNA AK057716 (BinCARD-1) is an incompletely spliced derivative of the gene product of C9orf89, whereas CARD19 (BinCARD-2) represents the properly spliced isoform, with conservation across diverse species. Immunoblotting revealed expression of CARD19 in T cells, but no evidence of BinCARD-1 expression, and microscopy demonstrated that endogenous CARD19 localizes to mitochondria. Although we"],"journal":["Cellular immunology"],"pubmed_title":["CARD19, the protein formerly known as BinCARD, is a mitochondrial protein that does not regulate Bcl10-dependent NF-κB activation after TCR engagement."],"pmcid":["PMC7484395"],"funding_grant_id":["U01 GM109887","R01 AI125552"],"pubmed_authors":["Rios KE","Maynard SK","Washington M","Schaefer BC","Paul S","Kashyap AK"],"additional_accession":[]},"is_claimable":false,"name":"CARD19, the protein formerly known as BinCARD, is a mitochondrial protein that does not regulate Bcl10-dependent NF-κB activation after TCR engagement.","description":"After T cell receptor (TCR) engagement, the CARD11-Bcl10-Malt1 (CBM) complex oligomerizes to transduce NF-κB activating signals. Bcl10 is then degraded to limit NF-κB activation. The cDNA AK057716 (BinCARD-1) was reported to encode a novel CARD protein that interacts with Bcl10 and modestly inhibits NF-κB activation. In a later study, a second isoform, BinCARD-2, was identified. Here, we report that the cDNA AK057716 (BinCARD-1) is an incompletely spliced derivative of the gene product of C9orf89, whereas CARD19 (BinCARD-2) represents the properly spliced isoform, with conservation across diverse species. Immunoblotting revealed expression of CARD19 in T cells, but no evidence of BinCARD-1 expression, and microscopy demonstrated that endogenous CARD19 localizes to mitochondria. Although we","dates":{"release":"2020-01-01T00:00:00Z","publication":"2020 Oct","modification":"2026-05-07T19:36:06.801Z","creation":"2022-02-11T11:41:25.268Z"},"accession":"S-EPMC7484395","cross_references":{"pubmed":["32763502"],"doi":["10.1016/j.cellimm.2020.104179"]}}