biostudies-literatureMirkheshti AShahid Beheshti University of Medical Sciences228https://www.ebi.ac.uk/biostudies/studies/S-EPMC7487602biostudies-literatureUnknown20(1)<h4>Background</h4>Bupivacaine, an amid-type local anesthetic, is widely used for clinical patients especially in pregnant women. In addition to neurotoxicity effect of bupivacaine, it can cross the placenta, accumulates in this tissue and retained in fetal tissues. Nevertheless, whether bupivacaine can cause neurotoxicity in fetus remains unclear. Hence, this study was design to investigate the effects of maternal bupivacaine use on fetus hippocampal cell apoptosis and the possible related mechanism.<h4>Methods</h4>On day 15 of pregnancy, sciatic nerve of pregnant wistar rat (180-200 g) were exposed by lateral incision of the right thigh and 0.2 ml of bupivacaine was injected. After their delivery, we randomly selected one male offspring of every mother. On day 30 after of their birth, thBMC anesthesiologyMaternal sciatic nerve administered bupivacaine induces hippocampal cell apoptosis in offspring.PMC748760215193Mirzaei MMemary ETajbakhsh AZaringhalam JLak EMirkheshti ABaniasadi MShakeri AfalseMaternal sciatic nerve administered bupivacaine induces hippocampal cell apoptosis in offspring.<h4>Background</h4>Bupivacaine, an amid-type local anesthetic, is widely used for clinical patients especially in pregnant women. In addition to neurotoxicity effect of bupivacaine, it can cross the placenta, accumulates in this tissue and retained in fetal tissues. Nevertheless, whether bupivacaine can cause neurotoxicity in fetus remains unclear. Hence, this study was design to investigate the effects of maternal bupivacaine use on fetus hippocampal cell apoptosis and the possible related mechanism.<h4>Methods</h4>On day 15 of pregnancy, sciatic nerve of pregnant wistar rat (180-200 g) were exposed by lateral incision of the right thigh and 0.2 ml of bupivacaine was injected. After their delivery, we randomly selected one male offspring of every mother. On day 30 after of their birth, th2020-01-01T00:00:00Z2020 Sep2025-04-07T00:39:58.357Z2020-09-18T07:07:40ZS-EPMC74876023289405410.1186/s12871-020-01143-2