biostudies-literatureUnknown26(34)Fernandez-Ramos D<h4>Background</h4>Arachidyl amido cholanoic acid (Aramchol) is a potent downregulator of hepatic stearoyl-CoA desaturase 1 (SCD1) protein expression that reduces liver triglycerides and fibrosis in animal models of steatohepatitis. In a phase IIb clinical trial in patients with nonalcoholic steatohepatitis (NASH), 52 wk of treatment with Aramchol reduced blood levels of glycated hemoglobin A1c, an indicator of glycemic control.<h4>Aim</h4>To assess lipid and glucose metabolism in mouse hepatocytes and in a NASH mouse model [induced with a 0.1% methionine and choline deficient diet (0.1MCD)] after treatment with Aramchol.<h4>Methods</h4>Isolated primary mouse hepatocytes were incubated with 20 μmol/L Aramchol or vehicle for 48 h. Subsequently, analyses were performed including Western blotWorld journal of gastroenterology5101-5117https://www.ebi.ac.uk/biostudies/studies/S-EPMC7495035biostudies-literatureArachidyl amido cholanoic acid improves liver glucose and lipid homeostasis in nonalcoholic steatohepatitis <i>via</i> AMPK and mTOR regulation.PMC7495035Lu SCvan Liempd SMGoni FMAzkargorta MSot JDelacruz-Villar LBilbao JSerrano-Macia MMato JMPagano MBizkarguenaga MAlonso CLopitz-Otsoa FMartinez-Chantar MLMosca LHayardeny LIruarrizaga-Lejarreta MTsvirkun DFernandez-Ramos DElortza FfalseArachidyl amido cholanoic acid improves liver glucose and lipid homeostasis in nonalcoholic steatohepatitis <i>via</i> AMPK and mTOR regulation.<h4>Background</h4>Arachidyl amido cholanoic acid (Aramchol) is a potent downregulator of hepatic stearoyl-CoA desaturase 1 (SCD1) protein expression that reduces liver triglycerides and fibrosis in animal models of steatohepatitis. In a phase IIb clinical trial in patients with nonalcoholic steatohepatitis (NASH), 52 wk of treatment with Aramchol reduced blood levels of glycated hemoglobin A1c, an indicator of glycemic control.<h4>Aim</h4>To assess lipid and glucose metabolism in mouse hepatocytes and in a NASH mouse model [induced with a 0.1% methionine and choline deficient diet (0.1MCD)] after treatment with Aramchol.<h4>Methods</h4>Isolated primary mouse hepatocytes were incubated with 20 μmol/L Aramchol or vehicle for 48 h. Subsequently, analyses were performed including Western blot2020-01-01T00:00:00Z2020 Sep2025-05-29T19:38:24.39Z2020-10-01T07:08:36ZS-EPMC74950353298211210.3748/wjg.v26.i34.5101