biostudies-literatureBilling AMEuropean Research CouncilFP7 Nanosciences, Nanotechnologies, Materials and new Production TechnologiesH2020 Leadership in Enabling and Industrial TechnologiesSeventh Framework ProgrammeDanish Centre for Nanosafety 24096-4110https://www.ebi.ac.uk/biostudies/studies/S-EPMC7498156biostudies-literatureUnknown14(4)Despite broad application of magnetic nanoparticles in biomedicine and electronics, only a few <i>in vivo</i> studies on biocompatibility are available. In this study, toxicity of magnetic metal oxide nanoparticles on the respiratory system was examined <i>in vivo</i> by single intratracheal instillation in mice. Bronchoalveolar lavage fluid (BALF) samples were collected for proteome analyses by LC-MS/MS, testing Fe₃O₄ nanoparticles doped with increasing amounts of cobalt (Fe₃O₄, CoFe₂O₄ with an iron to cobalt ratio 5:1, 3:1, 1:3, Co₃O₄) at two doses (54 μg, 162 μg per animal) and two time points (day 1 and 3 days postinstillation). In discovery phase, in-depth proteome profiling of a few representativeACS nanoFast and Robust Proteome Screening Platform Identifies Neutrophil Extracellular Trap Formation in the Lung in Response to Cobalt Ferrite Nanoparticles.PMC7498156720952320451646603310451Tang SVYWallin HVogel UChetwynd AJBerthing TBilling AMKnudsen KBKjeldsen FEllis LALynch IfalseFast and Robust Proteome Screening Platform Identifies Neutrophil Extracellular Trap Formation in the Lung in Response to Cobalt Ferrite Nanoparticles.Despite broad application of magnetic nanoparticles in biomedicine and electronics, only a few <i>in vivo</i> studies on biocompatibility are available. In this study, toxicity of magnetic metal oxide nanoparticles on the respiratory system was examined <i>in vivo</i> by single intratracheal instillation in mice. Bronchoalveolar lavage fluid (BALF) samples were collected for proteome analyses by LC-MS/MS, testing Fe₃O₄ nanoparticles doped with increasing amounts of cobalt (Fe₃O₄, CoFe₂O₄ with an iron to cobalt ratio 5:1, 3:1, 1:3, Co₃O₄) at two doses (54 μg, 162 μg per animal) and two time points (day 1 and 3 days postinstillation). In discovery phase, in-depth proteome profiling of a few representative2020-01-01T00:00:00Z2020 Apr2026-05-03T04:50:53.211Z2020-10-08T07:03:57ZS-EPMC74981563216728010.1021/acsnano.9b08818