biostudies-literatureColavita FMinistry of HealthEuropean Commission–Horizon 2020ofaa403https://www.ebi.ac.uk/biostudies/studies/S-EPMC7499768biostudies-literatureUnknown7(10)<h4>Background</h4>The pathogenesis of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection remains unclear. We report the detection of viral RNA from different anatomical districts and the antibody profile in the first 2 COVID-19 cases diagnosed in Italy.<h4>Methods</h4>We tested for SARS-CoV-2 RNA clinical samples, either respiratory and nonrespiratory (ie, saliva, serum, urine, vomit, rectal, ocular, cutaneous, and cervico-vaginal swabs), longitudinally collected from both patients throughout the hospitalization. Serological analysis was carried out on serial serum samples to evaluate IgM, IgA, IgG, and neutralizing antibody levels.<h4>Results</h4>SARS-CoV-2 RNA was detected since the early phase of illness, lasting over 2 weeks in both upper and lower respiratory tracOpen forum infectious diseasesVirological Characterization of the First 2 COVID-19 Patients Diagnosed in Italy: Phylogenetic Analysis, Virus Shedding Profile From Different Body Sites, and Antibody Response Kinetics.PMC7499768COVID 2020 12371675COVID-2020-12371817101003551101003544–CoNVatMarchioni LApollonio CFerraioli VBenigni MFelici LGiombini ELeone BCampioni PCasetti RPetrosillo NBiancone SAmendola APianura EVescovo TNoto RPasseri VScorzolini LPittalis SGarotto GMatusali GVenditti CAleo LBartolini BFerraro FAgrati CVulcano ACastilletti CCristofaro MTurchi FMacchione MRozera GDantimi CPalazzolo CPetrivelli ECarletti FPallini EDi Caro ASciamanna RFabeni LRueca MLalle EBrega CPetruccioli EMinosse CMariano ASelleri MSantagata CGiancola MLSanasi FIppolito GSelvaggi CFiorentini LProietti CDi Filippo SAlbarello FVincenti DPelliccioni FAntonini MMarani ADi Giuli CBranca MTruffa SScarcia SSberna GINMI COVID-19 Laboratory Team and INMI COVID-19 Study GroupMurachelli SScognamiglio PGruber CEMSarti SD'Abramo AArduini NCoen SCoppola AMeschi SAbbate ICannas AToffoletti ABibbo ASabatini RGarbuglia ARAbbonizio MACimini EGalli PPizzi DAscoli Bartoli TRaparelli FFrustaci ACuriale SDe Angelis GBordi LForbici FProta GNicastri EKhouri DStazi GDe Angelis ALucia APetrecchia ABerno GMassimino CCaravella ID'Arezzo SColavita FBevilacqua NDi Lorenzo RVaia FPisciotta MCaporale CCapobianchi MRMarini MCGraziano SStellitano CSchinina VCondello RCiaralli CAmadei GLapa DCarrara SGori CGiansante FSias CMontaldo CNeri SBordoni VGreci MCSacchi AMazzarelli ANisii CBiava MCiafrone LZambelli EBarbaro RLufrani FLepore LPetrone AChiappini RPuro VCanali MAlonzi TMarsella PRinonapoli GCorpolongo AValli MBLanini SMaritti MPetroni FDi Stefano FVairo FMessina FfalseVirological Characterization of the First 2 COVID-19 Patients Diagnosed in Italy: Phylogenetic Analysis, Virus Shedding Profile From Different Body Sites, and Antibody Response Kinetics.<h4>Background</h4>The pathogenesis of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection remains unclear. We report the detection of viral RNA from different anatomical districts and the antibody profile in the first 2 COVID-19 cases diagnosed in Italy.<h4>Methods</h4>We tested for SARS-CoV-2 RNA clinical samples, either respiratory and nonrespiratory (ie, saliva, serum, urine, vomit, rectal, ocular, cutaneous, and cervico-vaginal swabs), longitudinally collected from both patients throughout the hospitalization. Serological analysis was carried out on serial serum samples to evaluate IgM, IgA, IgG, and neutralizing antibody levels.<h4>Results</h4>SARS-CoV-2 RNA was detected since the early phase of illness, lasting over 2 weeks in both upper and lower respiratory trac2020-01-01T00:00:00Z2020 Oct2025-04-27T01:16:26.913Z2021-02-21T06:31:31ZS-EPMC74997683352708110.1093/ofid/ofaa403