biostudies-literatureUnknown20(1)Yao T<h4>Background</h4>We have previously found there was a small subpopulation of cells with cancer stem cell-like phenotype ALDH-1 in cervical cancer. Radiotherapy has been applied in most of the cervical cancer. However,the mechanisms underlying radioresistance still remained elusive. Our study is to explore whether ALDH+ cell promotes radioresistance by hypoxia.<h4>Methods</h4>Cells were respectively cultured in hypoxia and normoxia environment and analyzed for marker stability, and cell cycle distribution.<h4>Results</h4>Cell growth, apoptosis, cell cycle, sphere formation were affected by hypoxia. ALDH-1 and CHK2 were upregulated after hypoxia.<h4>Conclusions</h4>Here we show that ALDH-1 positive cells contribute to cervical carcinoma radioresistance through preferential activation of thBMC cancer891https://www.ebi.ac.uk/biostudies/studies/S-EPMC7499852biostudies-literatureALDH-1-positive cells exhibited a radioresistant phenotype that was enhanced with hypoxia in cervical cancer.PMC7499852Peng YHuang CYao YLi JLin RWeng XYao TLin ZfalseALDH-1-positive cells exhibited a radioresistant phenotype that was enhanced with hypoxia in cervical cancer.<h4>Background</h4>We have previously found there was a small subpopulation of cells with cancer stem cell-like phenotype ALDH-1 in cervical cancer. Radiotherapy has been applied in most of the cervical cancer. However,the mechanisms underlying radioresistance still remained elusive. Our study is to explore whether ALDH+ cell promotes radioresistance by hypoxia.<h4>Methods</h4>Cells were respectively cultured in hypoxia and normoxia environment and analyzed for marker stability, and cell cycle distribution.<h4>Results</h4>Cell growth, apoptosis, cell cycle, sphere formation were affected by hypoxia. ALDH-1 and CHK2 were upregulated after hypoxia.<h4>Conclusions</h4>Here we show that ALDH-1 positive cells contribute to cervical carcinoma radioresistance through preferential activation of th2020-01-01T00:00:00Z2020 Sep2025-05-31T23:16:42.254Z2020-09-25T07:03:26ZS-EPMC74998523294299610.1186/s12885-020-07337-8