biostudies-literatureFoster DJNational Cancer InstituteNCI NIH HHS2314-2326https://www.ebi.ac.uk/biostudies/studies/S-EPMC7513864biostudies-literatureUnknown19(18)TRIM71 is an important RNA-binding protein in development and disease, yet its direct targets have not been investigated globally. Here we describe a number of disease and developmentally-relevant TRIM71 RNA targets such as the <i>MBNL</i> family, <i>LIN28B, MDM2</i>, and <i>TCF7L2</i>. We describe a new role for TRIM71 as capable of positive or negative RNA regulation depending on the RNA target. We found that TRIM71 co-precipitated with IMP1 which could explain its multiple mechanisms of RNA regulation, as IMP1 is typically thought to stabilize RNAs. Deletion of the NHL domain of TRIM71 impacted its ability to bind to RNA and RNAs bound by congenital hydrocephalus-associated point mutations in the RNA-binding NHL domain of TRIM71 clustered closely with RNAs bound by the NHL deletion mutaCell cycle (Georgetown, Tex.)TRIM71 binds to IMP1 and is capable of positive and negative regulation of target RNAs.PMC7513864R35 CA232105R35CA232105Foster DJGregory RISlack FJChang HMHaswell JRfalseTRIM71 binds to IMP1 and is capable of positive and negative regulation of target RNAs.TRIM71 is an important RNA-binding protein in development and disease, yet its direct targets have not been investigated globally. Here we describe a number of disease and developmentally-relevant TRIM71 RNA targets such as the <i>MBNL</i> family, <i>LIN28B, MDM2</i>, and <i>TCF7L2</i>. We describe a new role for TRIM71 as capable of positive or negative RNA regulation depending on the RNA target. We found that TRIM71 co-precipitated with IMP1 which could explain its multiple mechanisms of RNA regulation, as IMP1 is typically thought to stabilize RNAs. Deletion of the NHL domain of TRIM71 impacted its ability to bind to RNA and RNAs bound by congenital hydrocephalus-associated point mutations in the RNA-binding NHL domain of TRIM71 clustered closely with RNAs bound by the NHL deletion muta2020-01-01T00:00:00Z2020 Sep2026-04-29T09:30:36.682Z2022-02-11T08:36:50.658ZS-EPMC75138643281659910.1080/15384101.2020.1804232