biostudies-literatureKrause JLDeutsche ForschungsgemeinschaftDeutsche Bundesstiftung Umwelt1116-1129https://www.ebi.ac.uk/biostudies/studies/S-EPMC7524388biostudies-literatureUnknown11(4)Diverse intestinal microbiota is frequently used in <i>in vitro</i> bioreactor models to study the effects of diet, chemical contaminations, or medication. However, the reproducible cultivation of fecal microbiota is challenging and the resultant communities behave highly dynamic. To approach the issue of reproducibility in <i>in vitro</i> models, we established an intestinal microbiota model community of reduced complexity, SIHUMIx, as a valuable model for <i>in vitro</i> use. The development of the SIHUMIx community was monitored over time with methods covering the cellular and the molecular level. We used microbial flow cytometry, intact protein profiling and terminal restriction fragment length polymorphism analysis to assess community structure. In parallel, we analyzed the functionalGut microbesFollowing the community development of SIHUMIx - a new intestinal <i>in vitro</i> model for bioreactor use.PMC7524388scholarshipPriority Program 1656Priority Program 2002Schaepe SSVon Bergen MFritz-Wallace KKleinsteuber SLiu ZHerberth GSchattenberg FRolle-Kampczyk UEngelmann BMueller SKrause JLJehmlich NfalseFollowing the community development of SIHUMIx - a new intestinal <i>in vitro</i> model for bioreactor use.Diverse intestinal microbiota is frequently used in <i>in vitro</i> bioreactor models to study the effects of diet, chemical contaminations, or medication. However, the reproducible cultivation of fecal microbiota is challenging and the resultant communities behave highly dynamic. To approach the issue of reproducibility in <i>in vitro</i> models, we established an intestinal microbiota model community of reduced complexity, SIHUMIx, as a valuable model for <i>in vitro</i> use. The development of the SIHUMIx community was monitored over time with methods covering the cellular and the molecular level. We used microbial flow cytometry, intact protein profiling and terminal restriction fragment length polymorphism analysis to assess community structure. In parallel, we analyzed the functional2020-01-01T00:00:00Z2020 Jul2025-04-04T12:22:42.061Z2020-10-29T08:13:49ZS-EPMC75243883191860710.1080/19490976.2019.1702431