{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Rivello F"],"funding":["Radboud University Nijmegen","Gellderland Province"],"pagination":["eaaz3849"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC7527228"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["6(40)"],"pubmed_abstract":["Despite their important role in metastatic disease, no general method to detect circulating stromal cells (CStCs) exists. Here, we present the Metabolic Assay-Chip (MA-Chip) as a label-free, droplet-based microfluidic approach allowing single-cell extracellular pH measurement for the detection and isolation of highly metabolically active cells (hm-cells) from the tumor microenvironment. Single-cell mRNA-sequencing analysis of the hm-cells from metastatic prostate cancer patients revealed that approximately 10% were canonical EpCAM<sup>+</sup> hm-CTCs, 3% were EpCAM<sup>-</sup> hm-CTCs with up-regulation of prostate-related genes, and 87% were hm-CStCs with profiles characteristic for cancer-associated fibroblasts, mesenchymal stem cells, and endothelial cells. Kaplan-Meier analysis shows t"],"journal":["Science advances"],"pubmed_title":["Probing single-cell metabolism reveals prognostic value of highly metabolically active circulating stromal cells in prostate cancer."],"pmcid":["PMC7527228"],"funding_grant_id":["Radboud Nanomedicine Alliance","Proeftuin Nanomedicine"],"pubmed_authors":["Mehra N","Smits M","Matula K","Huck WTS","Rivello F","Piruska A"],"additional_accession":[]},"is_claimable":false,"name":"Probing single-cell metabolism reveals prognostic value of highly metabolically active circulating stromal cells in prostate cancer.","description":"Despite their important role in metastatic disease, no general method to detect circulating stromal cells (CStCs) exists. Here, we present the Metabolic Assay-Chip (MA-Chip) as a label-free, droplet-based microfluidic approach allowing single-cell extracellular pH measurement for the detection and isolation of highly metabolically active cells (hm-cells) from the tumor microenvironment. Single-cell mRNA-sequencing analysis of the hm-cells from metastatic prostate cancer patients revealed that approximately 10% were canonical EpCAM<sup>+</sup> hm-CTCs, 3% were EpCAM<sup>-</sup> hm-CTCs with up-regulation of prostate-related genes, and 87% were hm-CStCs with profiles characteristic for cancer-associated fibroblasts, mesenchymal stem cells, and endothelial cells. Kaplan-Meier analysis shows t","dates":{"release":"2020-01-01T00:00:00Z","publication":"2020 Sep","modification":"2026-05-05T00:44:26.423Z","creation":"2020-10-29T08:12:22Z"},"accession":"S-EPMC7527228","cross_references":{"pubmed":["32998889"],"doi":["10.1126/sciadv.aaz3849"]}}