biostudies-literatureHao WTianjin UniversityNatural Science Foundation of Tianjin CityNational Natural Science Foundation of China24537-24545https://www.ebi.ac.uk/biostudies/studies/S-EPMC7528291biostudies-literatureUnknown5(38)Atomically precise Au₂₅(SG)₁₈ clusters have shown great promise in near-infrared II cerebrovascular imaging, X-ray imaging, and cancer radiotherapy due to their high atomic number, unique molecular-like electronic structure, and renal clearable properties. Therefore, it is important to study the in vivo toxicity of Au₂₅ clusters. Unfortunately, previous toxicological investigations focused on low injected doses (<100 mg kg^-1) and routine research methods, such as blood chemistry and biochemistry, which cannot reflect neurotoxicity or tiny changes in neural activity. In this work, in vivo neuroelectrophysiology of Au₂₅ clusters at ultrahigh injected doses (200, 300, and 500 mg kg^-1) was investigated. Local field potential shoACS omegaIn Vivo Neuroelectrophysiological Monitoring of Atomically Precise Au₂₅ Clusters at an Ultrahigh Injected Dose.PMC7528291U193210719JCZDJC34000918591018197174481471786Liu HMu XChen KZhang XDXin QHao WLiu SfalseIn Vivo Neuroelectrophysiological Monitoring of Atomically Precise Au₂₅ Clusters at an Ultrahigh Injected Dose.Atomically precise Au₂₅(SG)₁₈ clusters have shown great promise in near-infrared II cerebrovascular imaging, X-ray imaging, and cancer radiotherapy due to their high atomic number, unique molecular-like electronic structure, and renal clearable properties. Therefore, it is important to study the in vivo toxicity of Au₂₅ clusters. Unfortunately, previous toxicological investigations focused on low injected doses (<100 mg kg^-1) and routine research methods, such as blood chemistry and biochemistry, which cannot reflect neurotoxicity or tiny changes in neural activity. In this work, in vivo neuroelectrophysiology of Au₂₅ clusters at ultrahigh injected doses (200, 300, and 500 mg kg^-1) was investigated. Local field potential sho2020-01-01T00:00:00Z2020 Sep2025-04-04T07:40:44.273Z2025-04-04T07:40:44.273ZS-EPMC75282913301547110.1021/acsomega.0c03005