biostudies-literatureKim KRNational Research Foundation24790-24798https://www.ebi.ac.uk/biostudies/studies/S-EPMC7528338biostudies-literatureUnknown5(38)This study aims to suggest a simple migratory cell monitoring method in the Transwell system by utilizing retroreflective Janus microparticles (RJPs) as an optical probe. The RJP could be internalized on cells without compromising the cell viability and can be registered as bright spots within the cell body by inducing retroreflection from nonspectroscopic light sources. Conventional optical probes (e.g., fluorophores, chromogens, and nanoparticles) have been extensively studied and applied across diverse platforms (e.g., Boyden chamber, wound closing, and microfluidic chips) for understanding in vitro kinetic cell behavior. However, the complexities of running such platforms and setting up analytical instruments are limiting. In this regard, we aimed to demonstrate a modified Transwell miACS omegaNonspectroscopic Migratory Cell Monitoring Method Using Retroreflective Janus Microparticles.PMC75283382019M3D1A10789432019R1A6A1A11051471Jeong KYKim MSYoon HCKim JHKim KRLee DLee KWfalseNonspectroscopic Migratory Cell Monitoring Method Using Retroreflective Janus Microparticles.This study aims to suggest a simple migratory cell monitoring method in the Transwell system by utilizing retroreflective Janus microparticles (RJPs) as an optical probe. The RJP could be internalized on cells without compromising the cell viability and can be registered as bright spots within the cell body by inducing retroreflection from nonspectroscopic light sources. Conventional optical probes (e.g., fluorophores, chromogens, and nanoparticles) have been extensively studied and applied across diverse platforms (e.g., Boyden chamber, wound closing, and microfluidic chips) for understanding in vitro kinetic cell behavior. However, the complexities of running such platforms and setting up analytical instruments are limiting. In this regard, we aimed to demonstrate a modified Transwell mi2020-01-01T00:00:00Z2020 Sep2025-04-18T15:39:28.336Z2025-04-07T02:24:29.024ZS-EPMC75283383301549710.1021/acsomega.0c03454