biostudies-literatureUnknown11Chenebault CMinistère de l'Education Nationale, de l'Enseignement Supérieur et de la RechercheAgence Nationale de la RechercheCommissariat à l'Énergie Atomique et aux Énergies AlternativesCyanobacteria, the largest phylum of prokaryotes, perform oxygenic photosynthesis and are regarded as the ancestors of the plant chloroplast and the purveyors of the oxygen and biomass that shaped the biosphere. Nowadays, cyanobacteria are attracting a growing interest in being able to use solar energy, H<sub>2</sub>O, CO<sub>2</sub> and minerals to produce biotechnologically interesting chemicals. This often requires the introduction and expression of heterologous genes encoding the enzymes that are not present in natural cyanobacteria. However, only a handful of model strains with a well-established genetic system are being studied so far, leaving the vast biodiversity of cyanobacteria poorly understood and exploited. In this study, we focused on the robust unicellular cyanobacterium <i>Frontiers in microbiology586601https://www.ebi.ac.uk/biostudies/studies/S-EPMC7530172biostudies-literatureA Genetic Toolbox for the New Model Cyanobacterium <i>Cyanothece</i> PCC 7425: A Case Study for the Photosynthetic Production of Limonene.PMC7530172Chenebault CBuisson DASassi JFChauvat FStreckaite SGall ACassier-Chauvat CKammerscheit XDiaz-Santos EMarcon EBenzerara KIlioaia CGorgen SRobert BfalseA Genetic Toolbox for the New Model Cyanobacterium <i>Cyanothece</i> PCC 7425: A Case Study for the Photosynthetic Production of Limonene.Cyanobacteria, the largest phylum of prokaryotes, perform oxygenic photosynthesis and are regarded as the ancestors of the plant chloroplast and the purveyors of the oxygen and biomass that shaped the biosphere. Nowadays, cyanobacteria are attracting a growing interest in being able to use solar energy, H<sub>2</sub>O, CO<sub>2</sub> and minerals to produce biotechnologically interesting chemicals. This often requires the introduction and expression of heterologous genes encoding the enzymes that are not present in natural cyanobacteria. However, only a handful of model strains with a well-established genetic system are being studied so far, leaving the vast biodiversity of cyanobacteria poorly understood and exploited. In this study, we focused on the robust unicellular cyanobacterium <i>2020-01-01T00:00:00Z20202025-04-26T06:19:46.876Z2020-11-20T09:05:15ZS-EPMC75301723304210210.3389/fmicb.2020.586601