biostudies-literatureNontaleerak BScholarship for young scientists 2018, MUSCMahidol UniversityFaculty of Science, Mahidol UniversityThailand Research FundMOE | Office of the Higher Education CommissionChulabhorn Research Institutee01480-20https://www.ebi.ac.uk/biostudies/studies/S-EPMC7531977biostudies-literatureUnknown86(20)Reactive chlorine species (RCS), particularly hypochlorous acid (HOCl), are powerful antimicrobial oxidants generated by biological pathways and chemical syntheses. Pseudomonas aeruginosa is an important opportunistic pathogen that has adapted mechanisms for protection and survival in harsh environments, including RCS exposure. Based on previous transcriptomic studies of HOCl exposure in P. aeruginosa, we found that the expression of PA0565, or rcsA, which encodes an alkyl hydroperoxidase D-like protein, exhibited the highest induction among the RCS-induced genes. In this study, rcsA expression was dominant under HOCl stress and greatly increased under HOCl-related stress conditions. Functional analysis of RcsA showed that the distinguishing core amino acid residues Cys60, Cys63, and His67Applied and environmental microbiologyRoles of RcsA, an AhpD Family Protein, in Reactive Chlorine Stress Resistance and Virulence in Pseudomonas aeruginosa.PMC7531977MRG5980047Nontaleerak BWongsaroj LRomsang AMongkolsuk SDuang-Nkern JTrinachartvanit WfalseRoles of RcsA, an AhpD Family Protein, in Reactive Chlorine Stress Resistance and Virulence in Pseudomonas aeruginosa.Reactive chlorine species (RCS), particularly hypochlorous acid (HOCl), are powerful antimicrobial oxidants generated by biological pathways and chemical syntheses. Pseudomonas aeruginosa is an important opportunistic pathogen that has adapted mechanisms for protection and survival in harsh environments, including RCS exposure. Based on previous transcriptomic studies of HOCl exposure in P. aeruginosa, we found that the expression of PA0565, or rcsA, which encodes an alkyl hydroperoxidase D-like protein, exhibited the highest induction among the RCS-induced genes. In this study, rcsA expression was dominant under HOCl stress and greatly increased under HOCl-related stress conditions. Functional analysis of RcsA showed that the distinguishing core amino acid residues Cys60, Cys63, and His672020-01-01T00:00:00Z2020 Oct2025-04-26T10:14:01.692Z2022-02-09T10:53:57.596ZS-EPMC75319773280117110.1128/aem.01480-2010.1128/AEM.01480-20