{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["10(24)"],"submitter":["Han D"],"pubmed_abstract":["<b>Rationale:</b> Cell therapy for myocardial infarction is promising but largely unsuccessful in part due to a lack of mechanistic understanding. Techniques enabling identification of stem cell-specific proteomes <i>in situ</i> in the injured heart may shed light on how the administered cells respond to the injured microenvironment and exert reparative effects. <b>Objective:</b> To identify the proteomes of the transplanted mesenchymal stem cells (MSCs) in the infarcted myocardium, we sought to target a mutant methionyl-tRNA synthetase (MetRS<sup>L274G</sup>) in MSCs, which charges azidonorleucine (ANL), a methionine analogue and non-canonical amino acid, to tRNA and subsequently to nascent proteins, permitting isolation of ANL-labeled MSC proteomes from ischemic hearts by ANL-alkyne base"],"journal":["Theranostics"],"pagination":["11324-11338"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC7532665"],"repository":["biostudies-literature"],"pubmed_title":["Analysis of mesenchymal stem cell proteomes <i>in situ</i> in the ischemic heart."],"pmcid":["PMC7532665"],"pubmed_authors":["Mobley J","Yang J","Zhang E","Yang L","Zhang J","Lu D","Qiao A","Luo B","Jie C","Yan W","Boriboun C","Liu Y","Xu S","Qin G","Zhang C","Han D","Sun J","Yu Y"],"additional_accession":[]},"is_claimable":false,"name":"Analysis of mesenchymal stem cell proteomes <i>in situ</i> in the ischemic heart.","description":"<b>Rationale:</b> Cell therapy for myocardial infarction is promising but largely unsuccessful in part due to a lack of mechanistic understanding. Techniques enabling identification of stem cell-specific proteomes <i>in situ</i> in the injured heart may shed light on how the administered cells respond to the injured microenvironment and exert reparative effects. <b>Objective:</b> To identify the proteomes of the transplanted mesenchymal stem cells (MSCs) in the infarcted myocardium, we sought to target a mutant methionyl-tRNA synthetase (MetRS<sup>L274G</sup>) in MSCs, which charges azidonorleucine (ANL), a methionine analogue and non-canonical amino acid, to tRNA and subsequently to nascent proteins, permitting isolation of ANL-labeled MSC proteomes from ischemic hearts by ANL-alkyne base","dates":{"release":"2020-01-01T00:00:00Z","publication":"2020","modification":"2025-04-05T12:52:29.017Z","creation":"2020-10-29T09:20:18Z"},"accession":"S-EPMC7532665","cross_references":{"pubmed":["33042285"],"doi":["10.7150/thno.47893"]}}