biostudies-literatureUnknown10(24)Han D<b>Rationale:</b> Cell therapy for myocardial infarction is promising but largely unsuccessful in part due to a lack of mechanistic understanding. Techniques enabling identification of stem cell-specific proteomes <i>in situ</i> in the injured heart may shed light on how the administered cells respond to the injured microenvironment and exert reparative effects. <b>Objective:</b> To identify the proteomes of the transplanted mesenchymal stem cells (MSCs) in the infarcted myocardium, we sought to target a mutant methionyl-tRNA synthetase (MetRS^L274G) in MSCs, which charges azidonorleucine (ANL), a methionine analogue and non-canonical amino acid, to tRNA and subsequently to nascent proteins, permitting isolation of ANL-labeled MSC proteomes from ischemic hearts by ANL-alkyne baseTheranostics11324-11338https://www.ebi.ac.uk/biostudies/studies/S-EPMC7532665biostudies-literatureAnalysis of mesenchymal stem cell proteomes <i>in situ</i> in the ischemic heart.PMC7532665Mobley JYang JZhang EYang LZhang JLu DQiao ALuo BJie CYan WBoriboun CLiu YXu SQin GZhang CHan DSun JYu YfalseAnalysis of mesenchymal stem cell proteomes <i>in situ</i> in the ischemic heart.<b>Rationale:</b> Cell therapy for myocardial infarction is promising but largely unsuccessful in part due to a lack of mechanistic understanding. Techniques enabling identification of stem cell-specific proteomes <i>in situ</i> in the injured heart may shed light on how the administered cells respond to the injured microenvironment and exert reparative effects. <b>Objective:</b> To identify the proteomes of the transplanted mesenchymal stem cells (MSCs) in the infarcted myocardium, we sought to target a mutant methionyl-tRNA synthetase (MetRS^L274G) in MSCs, which charges azidonorleucine (ANL), a methionine analogue and non-canonical amino acid, to tRNA and subsequently to nascent proteins, permitting isolation of ANL-labeled MSC proteomes from ischemic hearts by ANL-alkyne base2020-01-01T00:00:00Z20202025-04-05T12:52:29.017Z2020-10-29T09:20:18ZS-EPMC75326653304228510.7150/thno.47893