{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Goglia AG"],"funding":["NIBIB NIH HHS","NCI NIH HHS","National Institutes of Health","NIGMS NIH HHS","National Science Foundation"],"pagination":["240-253.e6"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC7540725"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["10(3)"],"pubmed_abstract":["Complex, time-varying responses have been observed widely in cell signaling, but how specific dynamics are generated or regulated is largely unknown. One major obstacle has been that high-throughput screens are typically incompatible with the live-cell assays used to monitor dynamics. Here, we address this challenge by screening a library of 429 kinase inhibitors and monitoring extracellular-regulated kinase (Erk) activity over 5 h in more than 80,000 single primary mouse keratinocytes. Our screen reveals both known and uncharacterized modulators of Erk dynamics, including inhibitors of non-epidermal growth factor receptor (EGFR) receptor tyrosine kinases (RTKs) that increase Erk pulse frequency and overall activity. Using drug treatment and direct optogenetic control, we demonstrate that "],"journal":["Cell systems"],"pubmed_title":["A Live-Cell Screen for Altered Erk Dynamics Reveals Principles of Proliferative Control."],"pmcid":["PMC7540725"],"funding_grant_id":["DP2 EB024247","T32 GM007388","F30 CA206408"],"pubmed_authors":["Toettcher JE","Basta LP","Jena SG","Silbert J","Devenport D","Goglia AG","Wilson MZ"],"additional_accession":[]},"is_claimable":false,"name":"A Live-Cell Screen for Altered Erk Dynamics Reveals Principles of Proliferative Control.","description":"Complex, time-varying responses have been observed widely in cell signaling, but how specific dynamics are generated or regulated is largely unknown. One major obstacle has been that high-throughput screens are typically incompatible with the live-cell assays used to monitor dynamics. Here, we address this challenge by screening a library of 429 kinase inhibitors and monitoring extracellular-regulated kinase (Erk) activity over 5 h in more than 80,000 single primary mouse keratinocytes. Our screen reveals both known and uncharacterized modulators of Erk dynamics, including inhibitors of non-epidermal growth factor receptor (EGFR) receptor tyrosine kinases (RTKs) that increase Erk pulse frequency and overall activity. Using drug treatment and direct optogenetic control, we demonstrate that ","dates":{"release":"2020-01-01T00:00:00Z","publication":"2020 Mar","modification":"2025-04-04T01:45:45.012Z","creation":"2025-04-04T01:45:45.012Z"},"accession":"S-EPMC7540725","cross_references":{"pubmed":["32191874"],"doi":["10.1016/j.cels.2020.02.005"]}}