biostudies-literatureOsinubi AUniversity of Lagos Central Research Committee200906https://www.ebi.ac.uk/biostudies/studies/S-EPMC7540745biostudies-literatureUnknown7(9)Prolinamides are present in secondary metabolites and have wide-ranging biological properties as well as antimicrobial and cytotoxic activities. N-(4'-substituted phenyl)-l-prolinamides 4a-4w were synthesized in two steps, starting from the condensation of p-fluoronitrobenzene 1a-1b with l-proline 2a-2b, under aqueous-alcoholic basic conditions to afford N-aryl-l-prolines 3a-3c, which underwent amidation via a two-stage, one-pot reaction involving SOCl2 and amines, to furnish l-prolinamides in 20-80% yield. The cytotoxicities of 4a-4w against four human carcinoma cell lines (SGC7901, HCT-116, HepG2 and A549) were evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-dipRoyal Society open scienceSynthesis and in vitro anticancer activities of substituted N-(4'-nitrophenyl)-l-prolinamides.PMC7540745CRC No. 2015/25Gui COsinubi ABao XFamiloni OKong JIzunobi JAsekun OfalseSynthesis and in vitro anticancer activities of substituted N-(4'-nitrophenyl)-l-prolinamides.Prolinamides are present in secondary metabolites and have wide-ranging biological properties as well as antimicrobial and cytotoxic activities. N-(4'-substituted phenyl)-l-prolinamides 4a-4w were synthesized in two steps, starting from the condensation of p-fluoronitrobenzene 1a-1b with l-proline 2a-2b, under aqueous-alcoholic basic conditions to afford N-aryl-l-prolines 3a-3c, which underwent amidation via a two-stage, one-pot reaction involving SOCl2 and amines, to furnish l-prolinamides in 20-80% yield. The cytotoxicities of 4a-4w against four human carcinoma cell lines (SGC7901, HCT-116, HepG2 and A549) were evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-dip2020-01-01T00:00:00Z2020 Sep2026-05-09T03:30:24.25Z2020-10-29T09:31:19ZS-EPMC75407453304705110.1098/rsos.200906