{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Jiang YY"],"funding":["National Transgenic Research Project"],"pagination":["257"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC7541250"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["21(1)"],"pubmed_abstract":["Prime editing is a novel and universal CRISPR/Cas-derived precision genome-editing technology that has been recently developed. However, low efficiency of prime editing has been shown in transgenic rice lines. We hypothesize that enhancing pegRNA expression could improve prime-editing efficiency. In this report, we describe two strategies for enhancing pegRNA expression. We construct a prime editing vector harboring two pegRNA variants for W542L and S621I double mutations in ZmALS1 and ZmALS2. Compared with previous reports in rice, we achieve much higher prime-editing efficiency in maize. Our results are inspiring and provide a direction for the optimization of plant prime editors."],"journal":["Genome biology"],"pubmed_title":["Prime editing efficiently generates W542L and S621I double mutations in two ALS genes in maize."],"pmcid":["PMC7541250"],"funding_grant_id":["2019ZX08010003-001-011 and 2016ZX08009002"],"pubmed_authors":["Chen QJ","Lin Q","Lu MH","Zhang Y","Han XL","Wang XC","Chai YP","Zhou Y","Jiang YY","Zhang Q","Gao C"],"additional_accession":[]},"is_claimable":false,"name":"Prime editing efficiently generates W542L and S621I double mutations in two ALS genes in maize.","description":"Prime editing is a novel and universal CRISPR/Cas-derived precision genome-editing technology that has been recently developed. However, low efficiency of prime editing has been shown in transgenic rice lines. We hypothesize that enhancing pegRNA expression could improve prime-editing efficiency. In this report, we describe two strategies for enhancing pegRNA expression. We construct a prime editing vector harboring two pegRNA variants for W542L and S621I double mutations in ZmALS1 and ZmALS2. Compared with previous reports in rice, we achieve much higher prime-editing efficiency in maize. Our results are inspiring and provide a direction for the optimization of plant prime editors.","dates":{"release":"2020-01-01T00:00:00Z","publication":"2020 Oct","modification":"2025-04-04T22:04:35.658Z","creation":"2020-10-29T09:09:09Z"},"accession":"S-EPMC7541250","cross_references":{"pubmed":["33023639"],"doi":["10.1186/s13059-020-02170-5"]}}