biostudies-literatureTurano HFundação de Amparo à Pesquisa do Estado de São Pauloe00346-20https://www.ebi.ac.uk/biostudies/studies/S-EPMC7549359biostudies-literatureUnknown202(21)Multidrug resistance (MDR) is a serious threat to public health, making the development of new antimicrobials an urgent necessity. Pyocins are protein antibiotics produced by <i>Pseudomonas aeruginosa</i> strains to kill closely related cells during intraspecific competition. Here, we report an in-depth biochemical, microbicidal, and structural characterization of a new S-type pyocin, named S8. Initially, we described the domain organization and secondary structure of S8. Subsequently, we observed that a recombinant S8 composed of the killing subunit in complex with the immunity (ImS8) protein killed the strain PAO1. Furthermore, mutation of a highly conserved glutamic acid to alanine (Glu100Ala) completely inhibited this antimicrobial activity. The integrity of the H-N-H motif is probablyJournal of bacteriologyMolecular Structure and Functional Analysis of Pyocin S8 from Pseudomonas aeruginosa Reveals the Essential Requirement of a Glutamate Residue in the H-N-H Motif for DNase Activity.PMC75493592017/23839-72017/09443-32013/07937-8Domingos RMNetto LESLincopan NTurano HGomes FGarratt RCDegenhardt MFSOliveira CLPfalseMolecular Structure and Functional Analysis of Pyocin S8 from Pseudomonas aeruginosa Reveals the Essential Requirement of a Glutamate Residue in the H-N-H Motif for DNase Activity.Multidrug resistance (MDR) is a serious threat to public health, making the development of new antimicrobials an urgent necessity. Pyocins are protein antibiotics produced by <i>Pseudomonas aeruginosa</i> strains to kill closely related cells during intraspecific competition. Here, we report an in-depth biochemical, microbicidal, and structural characterization of a new S-type pyocin, named S8. Initially, we described the domain organization and secondary structure of S8. Subsequently, we observed that a recombinant S8 composed of the killing subunit in complex with the immunity (ImS8) protein killed the strain PAO1. Furthermore, mutation of a highly conserved glutamic acid to alanine (Glu100Ala) completely inhibited this antimicrobial activity. The integrity of the H-N-H motif is probably2020-01-01T00:00:00Z2020 Oct2026-05-01T16:31:15.685Z2022-02-09T12:51:59.514ZS-EPMC75493593281709810.1128/JB.00346-2010.1128/jb.00346-20