{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Liu Z"],"funding":["National Science Foundation of China"],"pagination":["1342-1351"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC7549633"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["17(9)"],"pubmed_abstract":["microRNAs (miRNA) are small non-coding RNAs that modulate the myriad biological activities by targeting genes, and many studies showed that miRNAs played a pivotal role in insect development. Here, we find that Bm-miRNA (miR-34) controls larval growth and wing morphology by targeting <i>BmE74</i> and <i>BmCPG4</i>. Overexpression of miR-34 in the whole body caused a smaller body size, partially displays deformed wings and venation defects in adults. Ablation of miR-34 by transgenic CRISPR/Cas9 technology resulted in a severe developmental delay during the larval stage. Moreover, we confirmed that miR-34 directly targeted <i>BmE74</i> and <i>BmCPG4</i> by using a dual luciferase reporter assay in HEK293T cells. Remarkably, loss-of-function of <i>BmCPG4</i> caused wing defects, which was sim"],"journal":["RNA biology"],"pubmed_title":["miR-34 regulates larval growth and wing morphogenesis by directly modulating ecdysone signalling and cuticle protein in <i>Bombyx mori</i>."],"pmcid":["PMC7549633"],"funding_grant_id":["31530072"],"pubmed_authors":["Yang X","Liu Z","Huang Y","Zhang X","Ling L","Luo X","Yang D","Xu J"],"additional_accession":[]},"is_claimable":false,"name":"miR-34 regulates larval growth and wing morphogenesis by directly modulating ecdysone signalling and cuticle protein in <i>Bombyx mori</i>.","description":"microRNAs (miRNA) are small non-coding RNAs that modulate the myriad biological activities by targeting genes, and many studies showed that miRNAs played a pivotal role in insect development. Here, we find that Bm-miRNA (miR-34) controls larval growth and wing morphology by targeting <i>BmE74</i> and <i>BmCPG4</i>. Overexpression of miR-34 in the whole body caused a smaller body size, partially displays deformed wings and venation defects in adults. Ablation of miR-34 by transgenic CRISPR/Cas9 technology resulted in a severe developmental delay during the larval stage. Moreover, we confirmed that miR-34 directly targeted <i>BmE74</i> and <i>BmCPG4</i> by using a dual luciferase reporter assay in HEK293T cells. Remarkably, loss-of-function of <i>BmCPG4</i> caused wing defects, which was sim","dates":{"release":"2020-01-01T00:00:00Z","publication":"2020 Sep","modification":"2025-04-26T00:09:20.351Z","creation":"2025-04-06T09:37:54.677Z"},"accession":"S-EPMC7549633","cross_references":{"pubmed":["32401141"],"doi":["10.1080/15476286.2020.1767953"]}}