{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Castro MM"],"funding":["NICHD NIH HHS","Conselho Nacional de Desenvolvimento Científico e Tecnológico","NIDDK NIH HHS","Coordenação de Aperfeiçoamento de Pessoal de Nível Superior","National Institutes of Health","FAPEMIG"],"pagination":["1779526"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC7549744"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["8(3)"],"pubmed_abstract":["Epithelial cells connect with each other by tight junctions (TJs) in several tissues. In epididymides, TJs proteins form the blood-epididymis barrier (BEB), which is crucial for male fertility. However, little is known about BEB morphological and physiological aspects in wild animals. This study examines the region-specific distribution pattern of TJs proteins in <i>D. rotundus'</i> epididymis, assessing their regulation in rainy and dry season. The expression of zonula occludens-1 (ZO-1), and claudins (Cldn)-1, -3, and -4 were evaluated by confocal immunofluorescence and ELISA analysis. Herein, ZO-1 was strictly expressed in TJs, whereas Cldns were expressed in TJs and basolateral membranes of epithelial cells. Their co-localization and intensity of expression varied in the epididymal reg"],"journal":["Tissue barriers"],"pubmed_title":["Distribution pattern of ZO-1 and claudins in the epididymis of vampire bats."],"pmcid":["PMC7549744"],"funding_grant_id":["R01 HD040793","DK097124","R01 DK097124","99999.011031/2013-01","PPM-00621-18","APQ-02514-16","HD040793","302088/2018-0","420077/2018-9"],"pubmed_authors":["Hill E","Neves CA","Kim B","Serrao JE","Breton S","Machado-Neves M","Castro MM","Games PD"],"additional_accession":[]},"is_claimable":false,"name":"Distribution pattern of ZO-1 and claudins in the epididymis of vampire bats.","description":"Epithelial cells connect with each other by tight junctions (TJs) in several tissues. In epididymides, TJs proteins form the blood-epididymis barrier (BEB), which is crucial for male fertility. However, little is known about BEB morphological and physiological aspects in wild animals. This study examines the region-specific distribution pattern of TJs proteins in <i>D. rotundus'</i> epididymis, assessing their regulation in rainy and dry season. The expression of zonula occludens-1 (ZO-1), and claudins (Cldn)-1, -3, and -4 were evaluated by confocal immunofluorescence and ELISA analysis. Herein, ZO-1 was strictly expressed in TJs, whereas Cldns were expressed in TJs and basolateral membranes of epithelial cells. Their co-localization and intensity of expression varied in the epididymal reg","dates":{"release":"2020-01-01T00:00:00Z","publication":"2020 Jul","modification":"2025-04-26T00:16:53.268Z","creation":"2022-02-10T15:19:53.042Z"},"accession":"S-EPMC7549744","cross_references":{"pubmed":["32552339"],"doi":["10.1080/21688370.2020.1779526"]}}