biostudies-literatureGong YSTU Scientific Research Foundation for TalentsGuangdong Provincial Special Fund for Modern Agriculture Industry Technology Innovation TeamsDepartment of Education of Guangdong ProvinceNational Natural Science Foundation of ChinaNatural Science Foundation of Guangdong ProvinceKey Special Project for Introduced Talents Team of Southern Marine Science and Engineering Guangdong Laboratory849-862https://www.ebi.ac.uk/biostudies/studies/S-EPMC7549984biostudies-literatureUnknown11(1)Programmed cell death 6 (PDCD6) is a well-known apoptosis regulator that is involved in the immunity of mammals. However, the effects of miRNA-mediated regulation of PDCD6 expression on apoptosis and virus infection in organisms, especially in marine invertebrates, have not been extensively explored. In this study, PDCD6 of mud crab (<i>Scylla paramamosain</i>) (<i>Sp</i>-PDCD6) was characterized. The results showed that <i>Sp</i>-PDCD6 contains five EF-hands domains and could suppress virus infection <i>via</i> apoptosis promotion. It also presented that <i>Sp</i>-PDCD6 was directly targeted by miR-9875 <i>in vitro</i> and <i>in vivo</i>, miR-9875 served as a positive regulator during the virus invasion. The findings indicated that the miR-9875-PDCD6 pathway possessed fundamental effects VirulencemiR-9875 functions in antiviral immunity by targeting PDCD6 in mud crab (<i>Scylla paramamosain</i>).PMC75499842018A0303070442019KJ1412017KQNCX0724187615231802341NTF18001GML2019ZD0606Ren XLin SLi SKong TGong YfalsemiR-9875 functions in antiviral immunity by targeting PDCD6 in mud crab (<i>Scylla paramamosain</i>).Programmed cell death 6 (PDCD6) is a well-known apoptosis regulator that is involved in the immunity of mammals. However, the effects of miRNA-mediated regulation of PDCD6 expression on apoptosis and virus infection in organisms, especially in marine invertebrates, have not been extensively explored. In this study, PDCD6 of mud crab (<i>Scylla paramamosain</i>) (<i>Sp</i>-PDCD6) was characterized. The results showed that <i>Sp</i>-PDCD6 contains five EF-hands domains and could suppress virus infection <i>via</i> apoptosis promotion. It also presented that <i>Sp</i>-PDCD6 was directly targeted by miR-9875 <i>in vitro</i> and <i>in vivo</i>, miR-9875 served as a positive regulator during the virus invasion. The findings indicated that the miR-9875-PDCD6 pathway possessed fundamental effects 2020-01-01T00:00:00Z2020 Dec2025-04-04T15:02:58.18Z2020-11-07T10:03:31ZS-EPMC75499843259729210.1080/21505594.2020.1787078