biostudies-literatureBechtner JGerman Ministry of Economics and Technology ,AiF, WiFöE1150https://www.ebi.ac.uk/biostudies/studies/S-EPMC7555045biostudies-literatureUnknown9(9)<i>Liquorilactobacillus</i> (<i>L.</i>) <i>hordei</i> (formerly <i>Lactobacillus hordei</i>) is one of the dominating lactic acid bacteria within the water kefir consortium, being highly adapted to survive in this environment, while producing high molecular weight dextrans from sucrose. In this work, we extensively studied the physiological response of <i>L. hordei</i> TMW 1.1822 to sucrose compared to glucose, applying label-free, quantitative proteomics of cell lysates and exoproteomes. This revealed the differential expression of 53 proteins within cellular proteomes, mostly associated with carbohydrate uptake and metabolism. Supported by growth experiments, this suggests that <i>L. hordei</i> TMW 1.1822 favors fructose over other sugars. The dextransucrase was expressed irrespectively Foods (Basel, Switzerland)Living the Sweet Life: How <i>Liquorilactobacillus hordei</i> TMW 1.1822 Changes Its Behavior in the Presence of Sucrose in Comparison to Glucose.PMC7555045AiF 19180 NBechtner JVogel RFKiening MJakob FLudwig CfalseLiving the Sweet Life: How <i>Liquorilactobacillus hordei</i> TMW 1.1822 Changes Its Behavior in the Presence of Sucrose in Comparison to Glucose.<i>Liquorilactobacillus</i> (<i>L.</i>) <i>hordei</i> (formerly <i>Lactobacillus hordei</i>) is one of the dominating lactic acid bacteria within the water kefir consortium, being highly adapted to survive in this environment, while producing high molecular weight dextrans from sucrose. In this work, we extensively studied the physiological response of <i>L. hordei</i> TMW 1.1822 to sucrose compared to glucose, applying label-free, quantitative proteomics of cell lysates and exoproteomes. This revealed the differential expression of 53 proteins within cellular proteomes, mostly associated with carbohydrate uptake and metabolism. Supported by growth experiments, this suggests that <i>L. hordei</i> TMW 1.1822 favors fructose over other sugars. The dextransucrase was expressed irrespectively 2020-01-01T00:00:00Z2020 Aug2026-04-07T17:25:53.711Z2020-10-29T10:29:10ZS-EPMC75550453282554710.3390/foods9091150