{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["21(18)"],"submitter":["Klafki HW"],"pubmed_abstract":["The ratio of amyloid precursor protein (APP)669-711 (Aβ-3-40)/Aβ1-42 in blood plasma was reported to represent a novel Alzheimer's disease biomarker. Here, we describe the characterization of two antibodies against the N-terminus of Aβ-3-x and the development and \"fit-for-purpose\" technical validation of a sandwich immunoassay for the measurement of Aβ-3-40. Antibody selectivity was assessed by capillary isoelectric focusing immunoassay, Western blot analysis, and immunohistochemistry. The analytical validation addressed assay range, repeatability, specificity, between-run variability, impact of pre-analytical sample handling procedures, assay interference, and analytical spike recoveries. Blood plasma was analyzed after Aβ immunoprecipitation by a two-step immunoassay procedure. Both mono"],"journal":["International journal of molecular sciences"],"pagination":["E6564"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC7555726"],"repository":["biostudies-literature"],"pubmed_title":["Development and Technical Validation of an Immunoassay for the Detection of APP669-711 (Aβ-3-40) in Biological Samples."],"pmcid":["PMC7555726"],"pubmed_authors":["Klafki HW","Beyer I","Jahn O","Knolker HJ","Vogelgsang J","Wiltfang J","Matzen A","Zampar S","Rohdenburg L","Oberstein TJ","Lachmann I","Rieper P","Wirths O","Osterloh D"],"additional_accession":[]},"is_claimable":false,"name":"Development and Technical Validation of an Immunoassay for the Detection of APP669-711 (Aβ-3-40) in Biological Samples.","description":"The ratio of amyloid precursor protein (APP)669-711 (Aβ-3-40)/Aβ1-42 in blood plasma was reported to represent a novel Alzheimer's disease biomarker. Here, we describe the characterization of two antibodies against the N-terminus of Aβ-3-x and the development and \"fit-for-purpose\" technical validation of a sandwich immunoassay for the measurement of Aβ-3-40. Antibody selectivity was assessed by capillary isoelectric focusing immunoassay, Western blot analysis, and immunohistochemistry. The analytical validation addressed assay range, repeatability, specificity, between-run variability, impact of pre-analytical sample handling procedures, assay interference, and analytical spike recoveries. Blood plasma was analyzed after Aβ immunoprecipitation by a two-step immunoassay procedure. Both mono","dates":{"release":"2020-01-01T00:00:00Z","publication":"2020 Sep","modification":"2025-04-06T12:18:55.912Z","creation":"2020-10-29T10:51:11Z"},"accession":"S-EPMC7555726","cross_references":{"pubmed":["32911706"],"doi":["10.3390/ijms21186564"]}}