biostudies-literatureCesa-Luna CVicerrectoría de Investigación y Estudios de Posgrado-Benemérita Universidad Autónoma de PueblaConsejo Nacional de Ciencia y TecnologíaDirección de Innovación y Transferencia de ConocimientoApoyo Redes del Programa para el Desarrollo Profesional Docentee0240545https://www.ebi.ac.uk/biostudies/studies/S-EPMC7561207biostudies-literatureUnknown15(10)The bacterial strain, EMM-1, was isolated from the rhizosphere of red maize ("Rojo Criollo") and identified as Pseudomonas protegens EMM-1 based on phylogenetic analysis of 16S rDNA, rpoB, rpoD, and gyrB gene sequences. We uncovered genes involved in the production of antimicrobial compounds like 2,4-diacetylphloroglucinol (2,4-DAPG), pyoluteorin, and lectin-like bacteriocins. These antimicrobial compounds are also produced by other fluorescent pseudomonads alike P. protegens. Double-layer agar assay showed that P. protegens EMM-1 inhibited the growth of several multidrug-resistant (MDR) bacteria, especially clinical isolates of the genera Klebsiella and β-hemolytic Streptococcus. This strain also displayed inhibitory effects against diverse fungi, such as Aspergillus, Botrytis, and FusariPloS oneGrowth inhibition of pathogenic microorganisms by Pseudomonas protegens EMM-1 and partial characterization of inhibitory substances.PMC7561207COL525704-VIEP2018100524554-VIEP2018100425788- VIEP20182015-2018: CA-262538125/2942722016-3; 2016-4Bustillos-Cristales MDRBaez AJuarez-Gonzalez VRQuintero-Hernandez VRivera-Urbalejo AGaytan PMunoz-Rojas JLlano-Villarreal RCAguayo-Acosta ACesa-Luna CfalseGrowth inhibition of pathogenic microorganisms by Pseudomonas protegens EMM-1 and partial characterization of inhibitory substances.The bacterial strain, EMM-1, was isolated from the rhizosphere of red maize ("Rojo Criollo") and identified as Pseudomonas protegens EMM-1 based on phylogenetic analysis of 16S rDNA, rpoB, rpoD, and gyrB gene sequences. We uncovered genes involved in the production of antimicrobial compounds like 2,4-diacetylphloroglucinol (2,4-DAPG), pyoluteorin, and lectin-like bacteriocins. These antimicrobial compounds are also produced by other fluorescent pseudomonads alike P. protegens. Double-layer agar assay showed that P. protegens EMM-1 inhibited the growth of several multidrug-resistant (MDR) bacteria, especially clinical isolates of the genera Klebsiella and β-hemolytic Streptococcus. This strain also displayed inhibitory effects against diverse fungi, such as Aspergillus, Botrytis, and Fusari2020-01-01T00:00:00Z20202026-05-03T20:36:48.4Z2020-10-29T12:32:14ZS-EPMC75612073305735110.1371/journal.pone.0240545