biostudies-literatureHahn LDeutsche ForschungsgemeinschaftInterdisciplinary Centre for Clinical Research within the Faculty of Medicine at the RWTH Aachen UniversityVon-Behring-Röntgen-StiftungE1949https://www.ebi.ac.uk/biostudies/studies/S-EPMC7564366biostudies-literatureUnknown9(9)The Th2 cytokine IL-13 is involved in biliary epithelial injury and liver fibrosis in patients as well as in animal models. The aim of this study was to investigate IL-13 as a therapeutic target during short term and chronic intrahepatic cholestasis in an <i>Abcb4-</i>knockout mouse model (<i>Abcb4</i>^-/-). Lack of IL-13 protected <i>Abcb4</i>^-/- mice transiently from cholestasis. This decrease in serum bile acids was accompanied by an enhanced excretion of bile acids and a normalization of fecal bile acid composition. In <i>Abcb4^-/-/IL-13</i>^-/- double knockout mice, bacterial translocation to the liver was significantly reduced and the intestinal microbiome resembled the commensal composition in wild type animals. In addition, 52-week-old <i>AbCellsIL-13 as Target to Reduce Cholestasis and Dysbiosis in <i>Abcb4</i> Knockout Mice.PMC7564366SFB1021 Z02KFO309 Z01KFO 217RO 957/10-1project O3-1RO 3714/4SFB/TRR57, projects P13 and Q360-0002SFB-TR84 project B08RO 957/11Mayatepek EBaier ADrebber UWeiskirchen RWeigel MHelmrich NWindhorst ARoeb EDomann ERath THahn LOlejniczak SSchmidt NEwers CChurin YHain TMollenkopf HJRoderfeld MHerebian DSteinhoff UWirtz SfalseIL-13 as Target to Reduce Cholestasis and Dysbiosis in <i>Abcb4</i> Knockout Mice.The Th2 cytokine IL-13 is involved in biliary epithelial injury and liver fibrosis in patients as well as in animal models. The aim of this study was to investigate IL-13 as a therapeutic target during short term and chronic intrahepatic cholestasis in an <i>Abcb4-</i>knockout mouse model (<i>Abcb4</i>^-/-). Lack of IL-13 protected <i>Abcb4</i>^-/- mice transiently from cholestasis. This decrease in serum bile acids was accompanied by an enhanced excretion of bile acids and a normalization of fecal bile acid composition. In <i>Abcb4^-/-/IL-13</i>^-/- double knockout mice, bacterial translocation to the liver was significantly reduced and the intestinal microbiome resembled the commensal composition in wild type animals. In addition, 52-week-old <i>Ab2020-01-01T00:00:00Z2020 Aug2026-04-14T23:20:28.552Z2020-10-30T08:35:14ZS-EPMC75643663284695410.3390/cells9091949