{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Wang T"],"funding":["National Institute of Diabetes and Digestive and Kidney Diseases","NIDDK NIH HHS","National Cancer Institute","NCI NIH HHS"],"pagination":["101629"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC7567049"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["23(10)"],"pubmed_abstract":["Madin-Darby canine kidney II (MDCKII) cells are widely used to study epithelial morphogenesis. To better understand this process, we performed time course RNA-seq analysis of MDCKII 3D cystogenesis, along with polarized 2D cells for comparison. Our study reveals a biphasic change in the transcriptome that occurs after the first cell cycle and coincides with lumen establishment. This change appears to be linked to translocation of β-catenin, supported by analyses with <i>AVL9</i>- and <i>DENND5A</i>-knockdown clones, and regulation by HNF1B, supported by ATAC-seq study. These findings indicate a qualitative change model for transcriptome remodeling during epithelial morphogenesis, leading to cell proliferation decrease and cell polarity establishment. Furthermore, our study reveals that act"],"journal":["iScience"],"pubmed_title":["A Qualitative Change in the Transcriptome Occurs after the First Cell Cycle and Coincides with Lumen Establishment during MDCKII Cystogenesis."],"pmcid":["PMC7567049"],"funding_grant_id":["R01 DK120510","R01 DK091530","R01 CA182093"],"pubmed_authors":["Peng X","Schmitz RJ","Urdy S","Zhao S","Wang T","Lu Z","Kwon SH","Mostov KE","Dalton S"],"additional_accession":[]},"is_claimable":false,"name":"A Qualitative Change in the Transcriptome Occurs after the First Cell Cycle and Coincides with Lumen Establishment during MDCKII Cystogenesis.","description":"Madin-Darby canine kidney II (MDCKII) cells are widely used to study epithelial morphogenesis. To better understand this process, we performed time course RNA-seq analysis of MDCKII 3D cystogenesis, along with polarized 2D cells for comparison. Our study reveals a biphasic change in the transcriptome that occurs after the first cell cycle and coincides with lumen establishment. This change appears to be linked to translocation of β-catenin, supported by analyses with <i>AVL9</i>- and <i>DENND5A</i>-knockdown clones, and regulation by HNF1B, supported by ATAC-seq study. These findings indicate a qualitative change model for transcriptome remodeling during epithelial morphogenesis, leading to cell proliferation decrease and cell polarity establishment. Furthermore, our study reveals that act","dates":{"release":"2020-01-01T00:00:00Z","publication":"2020 Oct","modification":"2026-05-03T20:37:11.502Z","creation":"2020-10-29T12:32:26Z"},"accession":"S-EPMC7567049","cross_references":{"pubmed":["33089114"],"doi":["10.1016/j.isci.2020.101629"]}}