biostudies-literatureUnknown11Feng XThe herb <i>Bolbostemma paniculatum</i> (Maxim) <i>Franquet</i> (Cucurbitaceae family), also known as Tu-Bei-Mu (TBM) in Chinese, has shown curative effects to treat several types of cancer as an adjunctive therapy. Thereby we intend to find its effect on the human hepatocellular carcinoma (HCC) and to understand the pharmacological mechanism behind it. In this study, an integrative serum pharmacology-based approach linking serum pharmacology and bioinformatics prediction was employed. Firstly, we used the serum taken introgastrically from the rats dministered by TBM aqueous bulb extract to culture the HCC cell line BEL-7404 and detect its anti-tumor effects. Secondly, the TBM putative targets were predicted using the ETCM database and known therapeutic targets of NPC were collected from tFrontiers in pharmacology01261https://www.ebi.ac.uk/biostudies/studies/S-EPMC7569155biostudies-literatureAn Integrative Serum Pharmacology-Based Approach to Study the Anti-Tumor Activity of <i>B. paniculatum</i> Aqueous Bulb Extract on the Human Hepatocellular Carcinoma Cell Line BEL-7404.PMC7569155Chao XFeng XWang YShi HMa GfalseAn Integrative Serum Pharmacology-Based Approach to Study the Anti-Tumor Activity of <i>B. paniculatum</i> Aqueous Bulb Extract on the Human Hepatocellular Carcinoma Cell Line BEL-7404.The herb <i>Bolbostemma paniculatum</i> (Maxim) <i>Franquet</i> (Cucurbitaceae family), also known as Tu-Bei-Mu (TBM) in Chinese, has shown curative effects to treat several types of cancer as an adjunctive therapy. Thereby we intend to find its effect on the human hepatocellular carcinoma (HCC) and to understand the pharmacological mechanism behind it. In this study, an integrative serum pharmacology-based approach linking serum pharmacology and bioinformatics prediction was employed. Firstly, we used the serum taken introgastrically from the rats dministered by TBM aqueous bulb extract to culture the HCC cell line BEL-7404 and detect its anti-tumor effects. Secondly, the TBM putative targets were predicted using the ETCM database and known therapeutic targets of NPC were collected from t2020-01-01T00:00:00Z20202026-04-07T22:35:15.567Z2020-11-02T08:00:48ZS-EPMC75691553312300210.3389/fphar.2020.01261