biostudies-literatureUnknown15(10)Brockmann M<h4>Introduction</h4>Bacterial pathogens are often involved in dermatitis in reptiles. Exact identification of reptile-specific but otherwise uncommon bacterial species may be challenging. However, identification is crucial to evaluate the importance of the detected bacterial species.<h4>Objective</h4>The aim of this study was to assess the number of aerobic bacterial isolates cultured from skin-derived samples of reptiles which were not reliably identified by Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS), and to determine their identity.<h4>Material and methods</h4>Routine bacterial diagnostics were performed on 235 skin samples, and 417 bacterial isolates were analysed by MALDI-TOF MS. The isolates were grouped into categories based on their PloS onee0240085https://www.ebi.ac.uk/biostudies/studies/S-EPMC7571677biostudies-literatureChallenges in microbiological identification of aerobic bacteria isolated from the skin of reptiles.PMC7571677Pees MGentil MBrockmann MMuller EMarschang REAupperle-Lellbach HHeusinger AfalseChallenges in microbiological identification of aerobic bacteria isolated from the skin of reptiles.<h4>Introduction</h4>Bacterial pathogens are often involved in dermatitis in reptiles. Exact identification of reptile-specific but otherwise uncommon bacterial species may be challenging. However, identification is crucial to evaluate the importance of the detected bacterial species.<h4>Objective</h4>The aim of this study was to assess the number of aerobic bacterial isolates cultured from skin-derived samples of reptiles which were not reliably identified by Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS), and to determine their identity.<h4>Material and methods</h4>Routine bacterial diagnostics were performed on 235 skin samples, and 417 bacterial isolates were analysed by MALDI-TOF MS. The isolates were grouped into categories based on their 2020-01-01T00:00:00Z20202026-04-14T23:20:12.956Z2020-10-30T08:35:04ZS-EPMC75716773307507710.1371/journal.pone.0240085