{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Yamano-Adachi N"],"funding":["Japan Agency for Medical Research and Development"],"pagination":["17612"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC7572389"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["10(1)"],"pubmed_abstract":["Chinese hamster (Cricetulus griseus) ovary-derived Chinese hamster ovary (CHO) cells are the most commonly used mammalian hosts for the industrial production of recombinant therapeutics because of their ability to fold, assemble, and perform post-translational modifications, such as glycosylation, on proteins. They are also valuable for their ability to grow in serum-free suspension cultures. In this study, we established a cell line derived from lung tissue of Chinese hamsters, named Chinese hamster lung (CHL)-YN cells. The biosafety of CHL-YN cells was confirmed by in vitro sterility testing, mycoplasma detection, and reverse transcriptase assays. One of the key characteristics of CHL-YN cells was their doubling time of 8.1 h in chemically defined culture medium; thus, they proliferate m"],"journal":["Scientific reports"],"pubmed_title":["Establishment of fast-growing serum-free immortalised cells from Chinese hamster lung tissues for biopharmaceutical production."],"pmcid":["PMC7572389"],"funding_grant_id":["18ae0101066h0001, 19ae0101066h0002","17ae0101003h005"],"pubmed_authors":["Yamano-Adachi N","Arishima R","Puriwat S","Omasa T"],"additional_accession":[]},"is_claimable":false,"name":"Establishment of fast-growing serum-free immortalised cells from Chinese hamster lung tissues for biopharmaceutical production.","description":"Chinese hamster (Cricetulus griseus) ovary-derived Chinese hamster ovary (CHO) cells are the most commonly used mammalian hosts for the industrial production of recombinant therapeutics because of their ability to fold, assemble, and perform post-translational modifications, such as glycosylation, on proteins. They are also valuable for their ability to grow in serum-free suspension cultures. In this study, we established a cell line derived from lung tissue of Chinese hamsters, named Chinese hamster lung (CHL)-YN cells. The biosafety of CHL-YN cells was confirmed by in vitro sterility testing, mycoplasma detection, and reverse transcriptase assays. One of the key characteristics of CHL-YN cells was their doubling time of 8.1 h in chemically defined culture medium; thus, they proliferate m","dates":{"release":"2020-01-01T00:00:00Z","publication":"2020 Oct","modification":"2025-04-04T09:15:59.987Z","creation":"2020-10-29T12:35:08Z"},"accession":"S-EPMC7572389","cross_references":{"pubmed":["33077772"],"doi":["10.1038/s41598-020-74735-0"]}}