biostudies-literatureYamano-Adachi NJapan Agency for Medical Research and Development17612https://www.ebi.ac.uk/biostudies/studies/S-EPMC7572389biostudies-literatureUnknown10(1)Chinese hamster (Cricetulus griseus) ovary-derived Chinese hamster ovary (CHO) cells are the most commonly used mammalian hosts for the industrial production of recombinant therapeutics because of their ability to fold, assemble, and perform post-translational modifications, such as glycosylation, on proteins. They are also valuable for their ability to grow in serum-free suspension cultures. In this study, we established a cell line derived from lung tissue of Chinese hamsters, named Chinese hamster lung (CHL)-YN cells. The biosafety of CHL-YN cells was confirmed by in vitro sterility testing, mycoplasma detection, and reverse transcriptase assays. One of the key characteristics of CHL-YN cells was their doubling time of 8.1 h in chemically defined culture medium; thus, they proliferate mScientific reportsEstablishment of fast-growing serum-free immortalised cells from Chinese hamster lung tissues for biopharmaceutical production.PMC757238918ae0101066h0001, 19ae0101066h000217ae0101003h005Yamano-Adachi NArishima RPuriwat SOmasa TfalseEstablishment of fast-growing serum-free immortalised cells from Chinese hamster lung tissues for biopharmaceutical production.Chinese hamster (Cricetulus griseus) ovary-derived Chinese hamster ovary (CHO) cells are the most commonly used mammalian hosts for the industrial production of recombinant therapeutics because of their ability to fold, assemble, and perform post-translational modifications, such as glycosylation, on proteins. They are also valuable for their ability to grow in serum-free suspension cultures. In this study, we established a cell line derived from lung tissue of Chinese hamsters, named Chinese hamster lung (CHL)-YN cells. The biosafety of CHL-YN cells was confirmed by in vitro sterility testing, mycoplasma detection, and reverse transcriptase assays. One of the key characteristics of CHL-YN cells was their doubling time of 8.1 h in chemically defined culture medium; thus, they proliferate m2020-01-01T00:00:00Z2020 Oct2025-04-04T09:15:59.987Z2020-10-29T12:35:08ZS-EPMC75723893307777210.1038/s41598-020-74735-0