{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Tyrrell MG"],"funding":["Stonehill Undergraduate Research Experience","Stonehill College Rev. Francis J. Hurley, C.S.C., Endowed Chair Research Fund","National Science Foundation"],"pagination":["17625"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC7572425"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["10(1)"],"pubmed_abstract":["Although cells of mushroom-producing fungi typically contain paired haploid nuclei (n + n), most Armillaria gallica vegetative cells are uninucleate. As vegetative nuclei are produced by fusions of paired haploid nuclei, they are thought to be diploid (2n). Here we report finding haploid vegetative nuclei in A. gallica at multiple sites in southeastern Massachusetts, USA. Sequencing multiple clones of a single-copy gene isolated from single hyphal filaments revealed nuclear heterogeneity both among and within hyphae. Cytoplasmic bridges connected hyphae in field-collected and cultured samples, and we propose nuclear migration through bridges maintains this nuclear heterogeneity. Growth studies demonstrate among- and within-hypha phenotypic variation for growth in response to gallic acid, a"],"journal":["Scientific reports"],"pubmed_title":["Mosaic fungal individuals have the potential to evolve within a single generation."],"pmcid":["PMC7572425"],"funding_grant_id":["IOB 0637623"],"pubmed_authors":["Torres L","Wilson S","Daly K","Peabody RB","Peabody C","Shafrir A","Doiron RJ","James-Pederson M","Flannery M","Lukason D","Hirst RG","Hernon T","Peabody DC","Bickford H","Churchill AC","Tyrrell MG","Fallavollita K","Seidel B","Haghighat D","Wiswell L","Salisbury K","Cabral P","Ramirez-Tapia JC","McKenna-Hoffman K","Presti L","Allan-Perkins E","Mondo E"],"additional_accession":[]},"is_claimable":false,"name":"Mosaic fungal individuals have the potential to evolve within a single generation.","description":"Although cells of mushroom-producing fungi typically contain paired haploid nuclei (n + n), most Armillaria gallica vegetative cells are uninucleate. As vegetative nuclei are produced by fusions of paired haploid nuclei, they are thought to be diploid (2n). Here we report finding haploid vegetative nuclei in A. gallica at multiple sites in southeastern Massachusetts, USA. Sequencing multiple clones of a single-copy gene isolated from single hyphal filaments revealed nuclear heterogeneity both among and within hyphae. Cytoplasmic bridges connected hyphae in field-collected and cultured samples, and we propose nuclear migration through bridges maintains this nuclear heterogeneity. Growth studies demonstrate among- and within-hypha phenotypic variation for growth in response to gallic acid, a","dates":{"release":"2020-01-01T00:00:00Z","publication":"2020 Oct","modification":"2025-06-01T01:35:41.12Z","creation":"2020-10-29T12:35:34Z"},"accession":"S-EPMC7572425","cross_references":{"pubmed":["33077756"],"doi":["10.1038/s41598-020-74679-5"]}}