biostudies-literaturePaulsen MRosetrees TrustMedical Research CouncilNational Heart and Lung InstituteWellcome Trust572747https://www.ebi.ac.uk/biostudies/studies/S-EPMC7573121biostudies-literatureUnknown11Infections with respiratory syncytial virus (RSV) occurs repeatedly throughout life because sustained, protective memory responses fail to develop. Why this occurs is not known. During RSV infection the recognition of the virus <i>via</i> the cytosolic RIG-I like receptors and signaling <i>via</i> the adaptor protein MAVS is crucial for mounting an innate immune response. However, if this signaling pathway is important for T cell responses during primary infection and during re-infection is not fully elucidated. We describe a second peak of pro-inflammatory mediators during the primary immune response to RSV that coincides with the arrival of T cells into the lung. This second peak of cytokines/chemokines is regulated differently than the early peak and is largely independent of signaling <i>via</i> MAVS. This was concurrent with <i>Mavs</i> ^-/- mice mounting a strong T cell response to primary RSV infection, with robust IFN-γ; and Granzyme B production. However, after RSV re-infection, <i>Mavs</i> ^-/- mice showed fewer CD4⁺ and CD8⁺ short term memory T cells and their capacity to produce IFN-γ; and Granzyme B, was decreased. In sum, cytosolic recognition of RSV is important not only for initiating innate anti-viral responses but also for generating or maintaining efficient, short term T cell memory responses.Frontiers in immunologyMAVS Deficiency Is Associated With a Reduced T Cell Response Upon Secondary RSV Infection in Mice.PMC75731211048073G0800311M370109058/Z/15/ZPinpathomrat NJohansson CKirsebom FCMPaulsen MVarese AfalseMAVS Deficiency Is Associated With a Reduced T Cell Response Upon Secondary RSV Infection in Mice.Infections with respiratory syncytial virus (RSV) occurs repeatedly throughout life because sustained, protective memory responses fail to develop. Why this occurs is not known. During RSV infection the recognition of the virus <i>via</i> the cytosolic RIG-I like receptors and signaling <i>via</i> the adaptor protein MAVS is crucial for mounting an innate immune response. However, if this signaling pathway is important for T cell responses during primary infection and during re-infection is not fully elucidated. We describe a second peak of pro-inflammatory mediators during the primary immune response to RSV that coincides with the arrival of T cells into the lung. This second peak of cytokines/chemokines is regulated differently than the early peak and is largely independent of signaling <i>via</i> MAVS. This was concurrent with <i>Mavs</i> ^-/- mice mounting a strong T cell response to primary RSV infection, with robust IFN-γ; and Granzyme B production. However, after RSV re-infection, <i>Mavs</i> ^-/- mice showed fewer CD4⁺ and CD8⁺ short term memory T cells and their capacity to produce IFN-γ; and Granzyme B, was decreased. In sum, cytosolic recognition of RSV is important not only for initiating innate anti-viral responses but also for generating or maintaining efficient, short term T cell memory responses.2020-01-01T00:00:00Z20202025-04-22T20:12:54.365Z2020-10-31T09:58:09ZS-EPMC75731213312315010.3389/fimmu.2020.572747