{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Arora S"],"funding":["National Institutes of Health","NIGMS NIH HHS","National Science Foundation"],"pagination":["12210-12213"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC7573786"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["56(81)"],"pubmed_abstract":["Methyllysine sites in proteins are recognized by an array of reader domains that mediate protein-protein interactions for controlling cellular processes. Herein, we engineer a chromodomain, an essential methyllysine reader, to carry 4-azido-l-phenylalanine (AzF) via amber suppressor mutagenesis and demonstrate its potential to bind and crosslink methylated proteins in human cells. We further develop a first-of-its kind chromodomain variant bearing two AzF units with enhanced crosslinking potential suitable for profiling the transient methyllysine interactome."],"journal":["Chemical communications (Cambridge, England)"],"pubmed_title":["Engineering a methyllysine reader with photoactive amino acid in mammalian cells."],"pmcid":["PMC7573786"],"funding_grant_id":["R01GM123234","R01 GM130752","MCB-1817692","R01 GM123234","R01GM130752"],"pubmed_authors":["Hinkelman K","Islam K","Arora S","Sappa S"],"additional_accession":[]},"is_claimable":false,"name":"Engineering a methyllysine reader with photoactive amino acid in mammalian cells.","description":"Methyllysine sites in proteins are recognized by an array of reader domains that mediate protein-protein interactions for controlling cellular processes. Herein, we engineer a chromodomain, an essential methyllysine reader, to carry 4-azido-l-phenylalanine (AzF) via amber suppressor mutagenesis and demonstrate its potential to bind and crosslink methylated proteins in human cells. We further develop a first-of-its kind chromodomain variant bearing two AzF units with enhanced crosslinking potential suitable for profiling the transient methyllysine interactome.","dates":{"release":"2020-01-01T00:00:00Z","publication":"2020 Oct","modification":"2026-04-29T10:24:21.442Z","creation":"2026-04-07T15:09:22.594Z"},"accession":"S-EPMC7573786","cross_references":{"pubmed":["32926023"],"doi":["10.1039/d0cc03814h"]}}